Skip Navigation
Search
College of Arts and Sciences
Biochemistry & Cell Biology
HomeFacultyFaculty ProfilesVitaly Citovsky

Vitaly Citovsky

citovsky

SUNY Distinguished Professor
Department of Biochemistry and Cell Biology

414 Life Sciences Building
Stony Brook University
Stony Brook, NY 11794-5215

Office Phone: 631-632- 9534
Lab Phones: 631-632-1015/1016/1955/1004
Fax: 631-632-8575 
E-mail: vitaly.citovsky@stonybrook.edu 

  • Research Description

    Our research focuses on three main projects: genetic transformation of plant cells by Agrobacterium, intercellular transport of plant viruses and plant cell proteins, and remodeling of plant chromatin by histone modifications.

    The first two projects utilize plant pathogens—which pirate the host cellular pathways for their life cycles—as molecular tools to study fundamental questions in plant biology. The third project examines how plant genes are regulated by histone-modifying corepressor complexes. 

    Plant Genetic Transformation

    In our studies of Agrobacterium, a unique bacterium capable of transfer of genetic material between prokaryotic and eukaryotic cells, we are identifying and characterizing the involvement of basic cellular systems—such as nuclear import machinery, targeted proteolysis machinery, targeting of multiprotein complexes to the cell chromatin, and DNA repair machinery—in the nuclear and intranuclear transport and integration of the invading T-DNA. Also, we are studying bacterial effectors that interact with these plant systems and may mimic some of their functions. We are especially interested in those effectors that are exported into the host cell and subvert its defense responses for the benefit of the invading pathogen. Consistent with the basic, evolutionarily-conserved nature of the host processes required for genetic transformation by Agrobacterium, we demonstrated that this plant pathogen can in fact genetically transform human cells. 

    Plant Intercellular Transport

    In our studies of intercellular movement of plant viruses, we discovered that viral genomes most likely travel between cells as subviral complexes composed mainly of the viral genomic molecule and the viral cell-to-cell movement protein. We are identifying and characterizing cellular proteins that interact with the viral movement protein and likely regulate the process of the viral transport through plant intercellular connections, the plasmodesmata. Specifically, we focus on the viral and host control of the plasmodesmal callose sphincters that can constrict and relax these cell-to-cell channels. 

    Chromatin Remodeling

    We are also studying the structure, composition, and function of plant corepressor complexes involved in histone modification and chromatin remodeling. Histone modification represents a universal mechanism for regulation of eukaryotic gene expression that underlies such diverse biological processes as restriction of expression of neuronal genes to neurons in mammals and control of flowering in plants. In animal cells, these chromatin modifications are effected by well-defined multiprotein complexes containing specific histone-modifying activities. In plants, information about composition of chromatin-modifying corepressor complexes is just beginning to emerge. We are systematically identifying protein components of plant corepressor complexes, study their interactions in vivo, analyze their effects on histone methylation, acetylation, and ubiquitination, and employ reverse genetics to characterize the target genes of the corepressor complexes and their roles in plant development and morphogenesis.

  • TabTotal Publications 159 Edited Books 3 Patents 3

    Selected Publications Since 2000

    1. Citovsky, V. & Zambryski, P. (2000) Systemic transport of RNA in plants. Trends Plant Sci. 5, 52-54. [pdf reprint]
    2. Rhee, Y., Tzfira, T., Chen, M.H., Waigmann, E., & Citovsky, V. (2000) Cell-to-cell movement of tobacco mosaic virus: enigmas and explanations. Mol. Plant Pathol. 1, 33-39. [pdf reprint]
    3. Tzfira, T., Rhee, Y., Chen, M.H., Kunik, T., & Citovsky, V. (2000) Nucleic acid transport in plant-microbe interactions: the molecules that walk through the walls.Annu. Rev. Microbiol. 54, 187-219. [pdf reprint]
    4. Tzfira, T. & Citovsky, V. (2000) From host recognition to T-DNA integration: the function of bacterial and plant genes in the Agrobacterium-plant cell interaction. Mol. Plant Pathol. 1, 201-212. [pdf reprint]
    5. Rhee, Y., Gurel, F., Gafni, Y., Dingwall, C., & Citovsky, V. (2000) A genetic system for detection of protein nuclear import and export. Nat. Biotechnol. 18, 433-437. [pdf reprint]
    6. Chen, M.H., Sheng, J., Hind, G., Handa, A., & Citovsky, V. (2000) Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell-to-cell movement. EMBO J. 19, 913-920. [pdf reprint]
    7. Waigmann, E., Chen, M.H., Bachmaier, R., Ghoshroy, S., & Citovsky, V. (2000) Regulation of plasmodesmal transport by phosphorylation of tobacco mosaic virus cell-to-cell movement protein. EMBO J. 19, 4875-4884. [pdf reprint]
    8. Kunik, T., Tzfira, T., Kapulnik, Y., Gafni, Y., Dingwall, C., & Citovsky, V. (2001) Genetic transformation of HeLa cells by Agrobacterium. Proc. Natl. Acad. Sci. USA  98, 1871-1876. [pdf reprint]
    9. Ueki, S. & Citovsky, V. (2001) Intercellular RNA transport in plants. Curr. Top.  Plant Biol. 2, 167-179.

    10. Tzfira, T. & Citovsky, V. (2001) Comparison between nuclear import of nopaline- and octopine-specific VirE2 protein of Agrobacterium in plant and animal cells. Mol. Plant Pathol. 2, 171-176. [pdf reprint]
    11. Tzfira, T., Vaidya, M., & Citovsky, V. (2001) VIP1, an Arabidopsis protein that interacts with Agrobacterium VirE2, is involved in VirE2 nuclear import and Agrobacterium infectivity. EMBOJ. 20, 3596-3607 . [pdf reprint]
    12. Ueki, S. & Citovsky, V. (2001) Inhibition of post transcriptional gene silencing by non-toxic concentrations of cadmium. Plant J. 28, 283-291. [pdf reprint]
    13. Ueki, S. & Citovsky, V. (2001) RNA commutes to work: regulation of plant gene expression by systemically transported RNA molecules. BioEssays 23, 1087-1090. [pdf reprint]
    14. Tzfira, T. & Citovsky, V. (2002) Partners-in-infection: host proteins involved in genetic transformation of plant cells by Agrobacterium. Trends Cell Biol. 12, 121-129. [pdf reprint]
    15. Ueki, S. & Citovsky, V. (2002) The systemic movement of a tobamovirus is inhibited by a cadmium-ion-induced glycine-rich protein. Nat. Cell Biol. 4, 478-485. Cover Article [pdf reprint]
    16. Tzfira, T., Vaidya, M., & Citovsky, V. (2002) Increasing plant susceptibility to Agrobacterium infection by overexpression of the Arabidopsis VIP1 gene. Proc. Natl. Acad. Sci. USA  99, 10435-10440. [pdf reprint]
    17. Zhu, Y., Nam, J., Humara, J.M., Mysore, K., Lee, L.Y., Cao, H., Valentine, L., Li, J., Kaiser, A., Kopecky, A., Hwang, H.H., Bhattacharjee, S., Rao, P., Tzfira, T., Rajagopal, J., Yi, H.C., Yadav, V.B.S., Crane, Y., Lin, K., Larcher, Y., Gelvin, M., Knue, M., Zhao, X., Davis, S., Kim, S.I., Kumar, C.T.R., Choi, Y.J., Hallan, V., Chattopadhyay, S., Sui, X., Ziemienowitz, A., Matthysse, A.G., Citovsky, V., Hohn, B., & Gelvin, S.B. (2003) Identification of Arabidopsis rat mutants. Plant Physiol.132, 494-505. [pdf reprint]
    18. Wu, X., Dinneny, J.R., Crawford, K.M., Rhee, Y., Citovsky, V., Zambryski, P.C., & Weigel, D. (2003) Models of intercellular transcription factor movement in the Arabidopsis apex. Development 130, 3735-3745. [pdf reprint]
    19. Chen, M.H. & Citovsky, V. (2003) Systemic movement of a tobamovirus requires host cell pectin methylesterase. Plant J. 35, 386-392. Cover Article [pdf reprint]
    20. Tzfira, T. & Citovsky, V. (2003) The Agrobacterium-plant cell interaction: taking biology lessons from a bug. Plant Physiol. 133, 943-947. [pdf reprint]
    21. Tzfira, T., Frankman, L., Vaidya, M., & Citovsky, V. (2003) Site-specific integration of Agrobacterium T-DNA via double-stranded intermediates. Plant Physiol. 133, 1011-1023. Cover Article [pdf reprint]
    22. Avivi, y., morad, v., ben- meir, h., zhao, j., kashkush, k., Tzfira, t., Citovsky, V., & Grafi, G. (2004) Reorganization of specific chromosomal domains and activation of silent genes in plant cells acquiring pluripotentiality. Dev. Dyn. 230, 12-22. [pdf reprint]
    23. Abu-Arish, A., Frenkiel-Krispin, D., Fricke, T., Tzfira, T., Citovsky, V., Grayer Wolf, S., & Elbaum, M. (2004) Three-dimensional reconstruction of Agrobacterium VirE2 protein with single-stranded DNA. J. Biol. Chem. 279, 25359-25363. [pdf reprint]
    24. Eulálio, A., Nunes-Correia, I., Carvalho, A.L., Faro, C., Citovsky, V., Simões, S., & Pedroso de Lima, M.C. (2004) Two African swine fever virus proteins derived from a common precursor exhibit different nucleocytoplasmic transport activities. J. Virol. 78, 9731-9739. [pdf reprint]
    25. Wang, Y., Tzfira, T., Gaba, V., Citovsky, V., Palukaitis, P. & Gal-On, A. (2004) Functional analysis of the cucumber mosaic virus 2b protein: pathogenicity and nuclear localization. J. Gen. Virol. 85, 3135-3147. [pdf reprint]
    26. Waigmann, E., Ueki, S., Trutnyeva, K., & Citovsky, V. (2004) The ins and outs of non-destructive cell-to-cell and systemic movement of plant viruses. Crit. Rev. Plant Sci. 23, 195-250. [pdf reprint]
    27. Tzfira, T., Li, J., Lacroix, B., & Citovsky, V. (2004) Agrobacterium T-DNA integration: molecules and models. Trends Genet. 20, 375-383. [pdf reprint]
    28. Citovsky , V., Kapelnikov , A., Oliel , S., Zakai, N., Rojas , M.R., Gilbertson , R.L., Tzfira , T., & Loyter , A. (2004) Protein interactions involved in nuclear import of the Agrobacterium VirE2 protein in vivo and in vitro. J. Biol. Chem. 279, 29528-29533. [pdf reprint]
    29. Tian, G.W., Mohanty, A., Chary, S.N., Li, S., Paap, B., Drakakaki, G., Kopec, C.D., Li, J., Ehrhardt, D., Jackson, D., Rhee, S.Y., Raikhel, N.V., & Citovsky, V. (2004) High-throughput fluorescent tagging of full-length Arabidopsis gene products in planta. Plant Physiol. 135, 25-38. Cover Article [pdf reprint]
    30. Tzfira, T., Vaidya, M., & Citovsky, V. (2004) Involvement of targeted proteolysis in plant genetic transformation by Agrobacterium. Nature 431, 87-92. [pdf reprint]
    31. Nuclear Import and Export in Plants and Animals (2005) (T. Tzfira & V. Citovsky, eds.) Eurekah.com and Kluwer Acad./Plenum Publ., 223 pages. Cover. [pdf reprint]
    32. Loyter, A., Rosenbluh, J., Zakai, N., Li, J., Kozlovsky, S.V., Tzfira, T., & Citovsky, V. (2005) The plant VirE2 interacting protein 1 . A molecular link between the Agrobacterium T-complex and the host cell chromatin? Plant Physiol. 138, 1318-1321. [pdf reprint]
    33. Tzfira, T., Tian, G.W., Lacroix, B., Vyas, S., Li, J., Leitner-Dagan, Y., Krichevsky, A., Taylor, T., Vainstein, A., & Citovsky, V. (2005) pSAT vectors: a modular series of plasmids for fluorescent protein tagging and expression of multiple genes in plants. Plant Mol. Biol. 57, 503-516. Cover Article [pdf reprint]
    34. Nelson, R.S. & Citovsky, V. (2005) Plant viruses: invaders of cells and pirates of cellular pathways. Plant Physiol. 138, 1809-1814. [pdf reprint]
    35. Chen, M.H., Tian, G.W., Gafni, Y., & Citovsky, V. (2005) Effects of calreticulin on viral cell-to-cell movement. Plant Physiol. 138, 1866-1876. Cover Article [pdf reprint]
    36. Lacroix, B., Tzfira, T., Vaidya, M., & Citovsky, V. (2005) The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformation.EMBO J. 24, 428-437. [pdf reprint]
    37. Ueki, S., & Citovsky, V. (2005) Control improves with age: systemic transport in plant embryos and adults. Proc. Natl. Acad. Sci. USA 102, 1817-1818. [pdf reprint]
    38. Li, J., Vaidya, M., White, C., Vainstein, A., Citovsky, V., & Tzfira, T. (2005) Involvement of KU80 in T-DNA integration in plant cells. Proc. Natl. Acad. Sci. USA 102, 19231-19236. [pdf reprint]
    39. Li, J., Krichevsky, A., Vaidya, M., Tzfira, T., & Citovsky, V. (2005) Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by Agrobacterium. Proc. Natl. Acad. Sci. USA 102, 5733-5738. [pdf reprint]
    40. Ueki, S. & Citovsky, V. (2005) Identification of an interactor of cadmium ion-induced glycine-rich protein involved in regulation of callose levels in plant vasculature.Proc. Natl. Acad. Sci. USA 102, 12089-12094. [pdf reprint]
    41. Eulálio, A., Nunes-Correia, I., Carvalho, A.L., Faro, C., Citovsky, V., Salas, J., Salas, M.L., Simões, S., & Pedroso de Lima, M.C. (2006) Nuclear export of African swine fever virus p37 protein occurs through two distinct pathways and is mediated by three independent signals. J. Virol. 80, 1393-1404. [pdf reprint]
    42. Tzfira, T., Kunik, T., Gafni, Y., & Citovsky, V. (2006) Agrobacterium protocols: mammalian cells. Methods Mol Biol. 344, 435-451. [pdf reprint]
    43. Tzfira, T. & Citovsky, V. (2006) Agrobacterium-mediated genetic transformation of plants: biology and biotechnology. Curr. Opin. Biotechnol. 17, 147-154. [pdf reprint]
    44. Lacroix, B., Li, J., Tzfira, T., & Citovsky, V. (2006) Will you let me use your nucleus? How Agrobacterium gets its T-DNA expressed in the host plant cell. Can. J. Physiol. Pharmacol. 84, 333-345. [pdf reprint]
    45. Krichevsky, A., Kozlovsky, S.V., Gafni, Y., & Citovsky, V. (2006) Nuclear import and export of plant viral proteins and genomes. Mol. Plant Pathol. 7, 131-146. Cover Article [pdf reprint]
    46. Lacroix, B., Tzfira, T., Vainstein, A., & Citovsky, V. (2006) A case of promiscuity: Agrobacterium’s endless hunt for new partners. Trends Genet. 22, 29-37. Cover Article. [pdf reprint]
    47. Tian, G.W., Chen, M.H., Zaltsman, A., & Citovsky, V. (2006) A pollen-specific pectin methylesterase involved in pollen tube growth. Dev. Biol. 294, 83-91. [pdf reprint]
    48. Ueki, S. & Citovsky, V. (2007) Spread throughout the plant: systemic transport of viruses. Plant Cell Monogr. 7, 85-118. [pdf reprint]
    49. Citovsky, V., Lee, L.Y., Vyas, S., Glick, E., Chen, M.H., Vainstein, A., Gafni, Y., Gelvin, S.B., & Tzfira, T. (2006) Identification of subcellular localization of interacting proteins by bimolecular fluorescence complementation in planta. J. Mol. Biol. 362, 1120-1131. [pdf reprint]
    50. Chakrabarty, R., Banerjee, R., Chung, S-M., Farman, M., Citovsky, V., Hogenhout, S. A., Tzfira, T., & Goodin, M. (2007) pSITE vectors for stable integration or transient expression of autofluorescent protein fusions in plants: probing Nicotiana benthamiana-virus interactions. Mol. Plant-Microbe Interact. 20, 740-750. [pdf reprint]
    51. Sandal, I., Saini, U., Lacroix, B., Bhattacharya, A., Ahuja, P.S., & Citovsky, V. (2007) Agrobacterium-mediated genetic transformation of tea leaf explants: effects of counteracting bactericidity of leaf polyphenols without loss of bacterial virulence. Plant Cell Rep. 26, 169-176. [pdf reprint]
    52. Zrachya, A., Glick, E., Levy, A., Arazi, T., Citovsky, V., & Gafni, Y. (2007) Suppressor of RNA silencing encoded by Tomato yellow leaf curl virus-Israel. Virology 358, 159-165. Cover Article. [pdf reprint]
    53. Anand, A., Krichevsky, A., Schornack, S., Lahaye, T., Tzfira, T., Citovsky, V., & Mysore, K.S. (2007) Arabidopsis VIRE2 INTERACTING PROTEIN2 is required for Agrobacterium T-DNA integration in plants.. Plant Cell 19, 1695-1708. [pdf reprint]
    54. Citovsky, V., Kozlovsky, S.V., Lacroix, B., Zaltsman, A., Dafni, M., Vyas, S., Tovkach, A., & Tzfira, T. (2007) Biological systems of the host cell involved in Agrobacterium infection. Cell. Microbiol. 9, 9-20. [pdf reprint]
    55. Krichevsky, A., Kozlovsky, S.V., Tian, G.W., Chen, M.H., Zaltsman, A., & Citovsky, V. (2007) How pollen tubes grow. Dev. Biol. 303, 405-420. [pdf reprint]
    56. Krichevsky, A., Gutgarts, H., Kozlovsky, S.V., Tzfira, T., Sutton, A., Sternglanz, R., Mandel, G., & Citovsky, V. (2007) C2H2 zinc finger-SET histone methyltransferase, is a plant-specific chromatin modifier. Dev. Biol. 303, 259-269. [pdf reprint]
    57. Krichevsky, A., Kozlovsky, S.V., Gutgarts, H., & Citovsky, V. (2007) Arabidopsis co-repressor complexes containing amino oxidase-like proteins and plant-specific histone methyltransferases. Plant Signal. Behav. 2, 174-177. [pdf reprint]
    58. Zaltsman, A., Yi, B.Y., Gafni, Y., & Citovsky, V. (2007) Yeast-plant coupled vector system for identification of nuclear proteins. Plant Physiol. 145, 1264-1271. Cover Article. [pdf reprint]
    59. Tzfira, T., Kozlovsky, S.V., & Citovsky, V. (2007) Advanced expression vector systems: new weapons for plant research and biotechnology. Plant Physiol. 145, 1087-1089. [pdf reprint]
    60. Agrobacterium (2008) (T. Tzfira & V. Citovsky, eds.) Springer, 735 pages. Cover. [pdf reprint]
    61. Glick, E., Zrachya, A., Levy, Y., Mett, A., Gidoni, D., Belausov, E., Citovsky, V., & Gafni, Y. (2008) Interaction with the host SGS3 is required for suppression of RNA silencing by the Tomato yellow leaf curl virus V2 protein. Proc. Natl. Acad. Sci. USA 105, 157-161. [pdf reprint]
    62. Lacroix, B., Kozlovsky, S.V., & Citovsky, V. (2008) Recent patents on Agrobacterium-mediated gene and protein transfer, for research and biotechnology. Recent Pat. Gene DNA Seq. 2, 69-81. [pdf reprint]
    63. Tzfira, T. & Citovsky, V. (2008) Probing interactions between plant virus movement proteins and nucleic acids. Methods Mol. Biol. 451, 293-316. [pdf reprint]
    64. Petrunia, I.V., Frolova, O., Komarova, T.V., Kiselev, S.L., Citovsky, V., & Dorokhov, Y. (2008) Agrobacterium tumefaciens-induced bacteraemia does not lead to reporter gene expression in mouse organs. PLoS ONE 3, e2352. [pdf reprint]
    65. Citovsky, V., Gafni, Y., & Tzfira, T. (2008) Localizing protein-protein interactions by bimolecular fluorescence complementation in planta. Methods 45, 196-206. [pdf reprint]
    66. Lacroix, B., Loyter, A., & Citovsky, V. (2008) Association of the Agrobacterium T-DNA-protein complex with plant nucleosomes. Proc. Natl. Acad. Sci. USA 105, 15429-15434. [pdf reprint]
    67. Orlova, I., Nagegowdaa, D.A., Kish, C.M., Gutensohn, M., Maeda, H. Varbanova, M., Fridman, E., Yamaguchi, S., Hanada, S., Kamiya, Y., Krichevsky, A., Citovsky, V., Pichersky, E., & Dudareva, N. (2009) Small subunit of geranyl diphosphate synthase modifies the chain length specificity of phylogenetically distant geranylgeranyl diphosphate synthase in planta. Plant Cell 21, 4002-4017. [pdf reprint]
    68. Krichevsky, A., Zaltsman, A., Kozlovsky, S.V., Tian, G.W., & Citovsky, V. (2009) Regulation of root elongation by histone acetylation in Arabidopsis. J. Mol. Biol. 385, 45-50. [pdf reprint]
    69. Lacroix, B. & Citovsky, V. (2009) Agrobacterium aiming for the host chromatin: host and bacterial proteins involved in interactions between T-DNA and plant nucleosomes. Communic. Integr. Biol. 2, 42-45. [pdf reprint]
    70. Tzfira, T. & Citovsky, V. (2009) Systems biology of plant-pathogen interactions. Semin. Cell Dev. Biol. 20, 1015-1016. [pdf reprint]
    71. Citovsky, V., Zaltsman, A., Kozlovsky, S.V., Gafni, Y., & Krichevsky, A. (2009) Proteasomal degradation in plant-pathogen interactions. Semin. Cell Dev. Biol. 20, 1048-1054. [pdf reprint]
    72. Ueki, S., Lacroix, B., Krichevsky, A., Lazarowitz, S.G., & Citovsky, V. (2009) Functional transient transformation of intact Arabidopsis plants by microbombardment. Nat. Protoc. 4, 71-77. [pdf reprint]
    73. Komarova, T.V., Schwartz, A.M., Frolova, O.Y., Zvereva, A.S., Gleba, Y.Y., Citovsky, V. & Dorokhov, Y.L. (2010) Pol II-directed non-coding RNAs suppress nuclear export of mRNA. Plant Mol. Biol. 74, 591-603.
    74. Zaltsman, A., Kozlovsky, S.V., Yasmin, F., & Citovsky, V. (2010) Plant defense pathways subverted by Agrobacterium for genetic transformation. Plant Signal. Behav.5, 1245-1248. [pdf reprint]
    75. Ueki, S., Meyers, B.L., Yasmin, F., & Citovsky, V. (2010) A cell-to-cell macromolecular transport assay in planta utilizing biolistic bombardment. J. Vis. Exp. 42, pii 2208. [pdf reprint]
    76. Bhattacharya, A., Sood, P., & Citovsky, V. (2010). The roles of plant phenolics in defense and communication during Agrobacterium and Rhizobium infection. Mol. Plant Pathol. 11, 705-719. [pdf reprint]
    77. Ueki, S., Spektor, R., Natale, D.M., & Citovsky, V. (2010) ANK, a host cytoplasmic receptor for the Tobacco mosaic virus cell-to-cell movement protein, down-regulates callose levels at plasmodesmata to control intercellular transport. PLoS Pathog. 6, e1001201. [pdf reprint]
    78. Krichevsky, A., Meyers, B.L., Vainstein, A., Maliga, P., & Citovsky, V. (2010) Autoluminescent plants. PLoS ONE 5, e15461. [pdf reprint]
    79. Zaltsman, A., Krichevsky, A., Loyter, A., & Citovsky, V. (2010) Agrobacterium induces expression of a host F-box protein required for tumorigenicity. Cell Host Microbe7, 197-209. [pdf reprint]
    80. Plant Transformation Technologies (2011) (N. Stewart, Jr., A. Touraev, V. Citovsky, & T. Tzfira, eds.) Wiley-Blackwell, 296 pages. 
    81. Magori, S. & Citovsky, V. (2011) Hijacking of the host SCF ubiquitin ligase machinery by plant pathogens. Front. Plant Sci. 2, 87. [pdf reprint]
    82. Zavaliev, R., Ueki, S., Epel, B.L., & Citovsky, V. (2011) Biology of callose (ß-1,3-glucan) turnover at plasmodesmata. Protoplasma 248, 117-130. [pdf reprint]
    83. Ueki, S. & Citovsky, V. (2011) Protein membrane overlay assay: a protocol to test interaction between soluble and insoluble proteins in vitro. J. Vis. Exp. 54, e2961. [pdf reprint]
    84. Magori, S. & Citovsky, V. (2011) Epigenetic control of Agrobacterium T-DNA integration. Biochim. Biophys. Acta 1809, 388-394. [pdf reprint]
    85. Ueki, S. & Citovsky, V. (2011) To gate, or not to gate: regulatory mechanisms for intercellular protein transport and virus movement in plants. Mol. Plant 4, 782-793. [pdf reprint]
    86. Lacroix, B. & Citovsky, V. (2011) Extracellular VirB5 enhances T-DNA transfer from Agrobacterium to the host plant. PLOS ONE 6, e25578. [pdf reprint]
    87. Krichevsky, A., Zaltsman, A., Lacroix, B., & Citovsky, V. (2011) Involvement of KDM1C histone demethylase-OTLD1 otubain-like histone deubiquitinase complexes in plant gene repression. Proc. Natl. Acad. Sci. USA 108, 11157-11162. [pdf reprint]
    88. Magori, S. & Citovsky, V. (2011) Agrobacterium counteracts host-induced degradation of its F-box protein effector. Sci. Signal. 4, ra69. [pdf reprint]
    89. Mansfield, J., Genin, S., Magori, S., Citovsky, V., Sriariyanum, M., Ronald, P., Dow, M., Verdier, V., Beer, S.V., Machado, M.A., Toth, I., Salmond, G., & Foster, G.D. (2012) Top 10 plant bacteria in molecular plant pathology. Mol. Plant Pathol. 13, 614-629. [pdf reprint]
    90. Magori, S. & Citovsky, V. (2012) The role of the ubiquitin-proteasome system in Agrobacterium-mediated genetic transformation of plants. Plant Physiol. 160, 65-71. [pdf reprint]
    91. Ueki, S., Magori, S., Lacroix, B., & Citovsky, V. (2013) Transient gene expression in plants by biolistic DNA delivery. Methods Mol. Biol. 940, 17-26. [pdf reprint]
    92. Lacroix, B. & Citovsky, V. (2013) The roles of host plant factors in Agrobacterium-mediated genetic transformation. Int. J. Dev. Biol., in press.[pdf reprint]
    93. Lacroix, B. & Citovsky, V. (2013) Agrobacterium. In Brenner’s Encyclopedia of Genetics Second Edition (S. Maloy & K. Hughes, eds.), Elsevier, pp. 52-54. [pdf reprint]
    94. Lacroix, B. & Citovsky, V. (2013) Crown Gall Tumors. In Brenner’s Encyclopedia of Genetics Second Edition (S. Maloy & K. Hughes, eds.), Elsevier, pp. 236-239. [pdf reprint]
    95. Lacroix, B. & Citovsky, V. (2013) A mutation in negative regulator of basal resistance WRKY17 of Arabidopsis increases susceptibility to Agrobacterium-mediated transient genetic transformation. F1000 Research 2, 33. [pdf reprint]
    96. Zalepa-King, L. & Citovsky, V. (2013) A plasmodesmal glycosyltransferase-like protein. PLOS ONE 8, e58025.[pdf reprint]
    97. Lacroix, B. & Citovsky, V. (2013) Characterization of VIP1 activity as a transcriptional regulator in vitro and in planta. Sci. Rep. 3, 2440. [pdf reprint]
    98. Zaltsman, A., Lacroix, B., Gafni, Y., & Citovsky, V. (2013) Disassembly of synthetic Agrobacterium T-DNA-protein complexes via the host SCFVBF ubiquitin-ligase complex pathway. Proc. Natl. Acad. Sci. USA 110, 169-174. [pdf reprint]
    99. García-Cano, E., Zaltsman, A., & Citovsky, V. (2014) Assaying proteasomal degradation in a cell-free system in plants. J. Vis. Exp. 85, e51293. [pdf reprint]
    100. Ueki, S. & Citovsky, V. (2014) Plasmodesmata-associated proteins: can we see the whole elephant? Plant Signal. Behav. 9, e27899. [pdf reprint]
    101. Lacroix, B., Gizatullina, D.I., Babst, B.A., Gifford, A.N., & Citovsky, V. (2014) Agrobacterium T-DNA-encoded protein Atu6002 interferes with the host auxin response. Mol. Plant Pathol. 15, 275-283. [pdf reprint]
    102. Lacroix, B. & Citovsky, V. (2014) Plant genetic engineering: changing the world by advancing agriculture and research. In Discoveries in Modern Science: Exploration, Invention, Technology (J. Trefil, ed.), Macmillan, in press. 
    Patents
    1. Citovsky, V. (1998) “Protein-Mediated Nuclear Import of DNA”. U.S. patent # 5,831,020. [pdf reprint]
    2. Rhee, Y. & Citovsky, V. (2005) “Genetic Assay for Protein Nuclear Transport”. U.S. patent # 6,902,886. [pdf reprint]
    3. Tzfira, T. & Citovsky, V. (2010) “Increasing Host Plant Susceptibility to Agrobacterium Infection by Overexpression of the Arabidopsis VIP1 Gene”. U.S. patent # 7,659,447. [pdf reprint]
  • Journal and Book Covers

    The gateways to disease

    International Innovation Magazine

    2013

    		 Plant Physiology

    Plant Physiology

    September 2012

    Science

    Science Signaling

    October 2011

    Plant Transformation Technologies

    Plant Transformation Technologies

    Neal Stewart, Alisher Touraev, Vitaly Citovsky and Tzvi Tzfira Editors

    Wiley-Blackwell
    2011
     

    Seminars in Cell & Developmental Biology

    Seminars in Cell & Developmental Biology

    Systems Biology of Plant-Pathogen Interactions
    Vitaly Citovsky & Tzvi Tzfira Guest Editors

    Elsevier 
    Vol. 20, Issue 9
    December 2009

    		  

    Agrobacterium

    Agrobacterium
    From Biology to Biotechnology

    Tzvi Tzfira and Vitaly Citovsky Editors

    Springer
    2008
     

    Plant Physiology

    Plant Physiology

    Vol. 145 No. 4
    December 2007

    Plant Physiology

    Virology

    Vol. 35, No. 1

     

    Trends in Genetics

    Trends in Genetics

    Vol. 22 No. 1
    January 2006

    Molecular Plant Pathology

    Molecular Plant Pathology

    Vol. 7 Issue 2
    March 2006

     
     

    Plant Physiology

    Plant Physiology

    Vol. 138 No. 4
    August 2005

    		  

    Plant Molecular Biology

    Plant Molecular Biology

    Vol. 57 No. 4
    March 2005
     

     nuclear

    Nuclear Import and Export in Plants and Animals

    Tzvi Tzfira and Vitaly Citovsky Editors

    Landes Bioscience
    Kluwer Academic/Plenum Publishers
    2005

    Plant Physiology

    Plant Physiology

    Vol. 135 No. 1
    May 2004

     
     

    Plant Physiology

    Plant Physiology

    Vol. 133 No. 3
    November 2003

    		  

    The Plant Journal

    The Plant Journal

    Vol. 35 No. 3
    August 2003
    nature

    Nature Cell Biology

    Vol. 4 No. 7
    July 2002

    		  

    Science

    Science

    Vol. 256
    26 June 1992
  • CV
  • Lab Member
    Current Postdocs: 

    Elena Garcia, biomelena@hotmail.com

    Ido Keren, idokeren@gmail.com

    Benoit Lacroix, benoit.lacroix@stonybrook.edu

    Renhong Wui, renhong.wu@gmail.com

    Cheng Yuan, genesilence@gmail.com 

     

    Current Sabbatical Scholars: 

    Sondra Lazarowitz, sandy.lazarowitz@cornell.edu (Cornell University, Ithaca, NY)

  • Links