Gordon T. Taylor
Division Head, Marine Sciences
Director of the NAno-Raman Molecular Imaging Laboratory (NARMIL)
- University of Southern California
Marine molecular microbiology, interests in microbial ecology, plankton trophodynamics, anoxia, hypoxia, biogeochemistry, single cell analysis, Raman Microspectrometry and Atomic Force Microscopy
My research efforts have mainly focused on (i) microbial mediation of biogeochemical processes (particularly carbon cycling), (ii) food web interactions among microorganisms (bacteria, protozoans, algae and viruses), and (iii) and linking ecological function to specific microbial community members. Microbiological and chemical exchange processes across interfaces, such as oxic/anoxic, solid/water and air/water boundaries have also been a particular focus of our group.
Microbiological processes are intimately linked to the fate of carbon in the ocean and are responsive to climatic changes. As founding members of the NSF-funded CARIACO Ocean Time Series Program (1995-2017), our team’s research in the Cariaco Basin has improved understanding of current carbon cycling dynamics in the southern Caribbean Sea on the continental margin of Venezuela. For example, our studies have documented a >1°C rise in sea surface temperatures, declines in upwelling, nutrient supply, plankton productivity and pH as well as an ecosystem state changes (Taylor et al. 2012, 2018; Astor et al. 2013; Scranton et al. 2015). Results from this international, multi-institutional interdisciplinary project are also being used to better interpret ocean conditions and climate in the geologic past in order to better predict the future. To learn more about this program, visit its website or refer to some of the publications listed below.
Beyond being intrinsically fascinating, the Cariaco Basin serves as a model for other oxygen-depleted bodies of water (e.g., the Gulf of Mexico “Dead Zone”, Long Island Sound, the Black Sea, the Baltic and Mediterranean Deeps, major Oxygen Minimum Zones, and fjords), which are becoming more widespread geographically as climate changes and as human populations impact coastal waters, a phenomenon referred to as ocean deoxygenation. Our group has focused on microbial dynamics, biogeochemistry and transformations of organic materials transported from oxic to anoxic waters. We are particularly keen to understand the process of chemoautotrophy. The reason being that chemoautotrophic carbon fixation is globally important to food webs and can link carbon, nitrogen and sulfur cycles. In my lab, we combine traditional microbial ecological and geochemical measurements with modern molecular techniques, such as SSU rRNA libraries, stable isotope probing (SIP), fluorescence in situ hybridization (FISH), and quantitative PCR of functional genes and their expression, to unravel the interplay between chemical gradients, elemental cycling and microbial population dynamics.
Over the years, our holy grail has been to understand the jobs of specific microbial populations across wide-ranging geochemical seascapes. Making such linkages improves inferences about the role of specific microorganisms discovered within massive genomic databases. To that end, we have built an exciting new analytical facility within SoMAS – the NAno-Raman Molecular Imaging Laboratory (opened Jan 2014), through NSF’s Major Research Instrumentation funding. NARMIL houses a novel, state-of-the-art Renishaw inVia confocal laser Raman microspectrometer which permits 3-D mapping of biomolecules, minerals, and synthetic materials, while also providing fluorescent, bright field and dark field images. By combining SIP, phylogenetic FISH probes and Raman mapping, we determine identity of individual cells and their activity in assimilating particular isotope labels. NARMIL also houses a Bruker Innova Atomic Force Microscope (AFM), which produces topographic images comparable to electron microscopy, but under standard lab conditions. By coupling the AFM and Raman instruments, we can focus the laser beam on the AFM tip and produce nanometers-scale chemical maps of specimen surfaces. NARMIL provides SoMAS with unique and powerful analytical tools with countless applications throughout the natural sciences, biomedicine, and engineering. We are enthusiastically exploring new research frontiers with these novel capabilities! To learn more about our facility, please visit: http://you.stonybrook.edu/nanoraman/.
Cohen AB, Novkov-Bloom A, Wesselborg C, Yagudaeva M, Taylor GT. (2021) Fluorescence in situ hybridization in natural systems with Cyanobacteria blooms: procedural artifacts and potential solutions. Limnology and Oceanogrpahy: Methods. doi: 10.1002/lom3.10437
Medina Faull LE, Zaliznyak T, and Taylor GT. (2021) Assessing diversity, abundance, and mass of microplastics (~ 1–300 μm) in aquatic systems Limnology and Oceanography: Methods. doi: 10.1002/lom3.10430
Nayfach S, Roux S, Seshadri R, Udwary D, Varghese N Schulz F, IMG/M Data Consortium, et al. (2020). A Genomic Catalogue of Earth’s Microbiomes. Nature Biotechnology https://doi.org/10.1038/s41587-020-0718-6 .
Suter EA, Pachiadaki MG, Montes E, Edgcomb VP, Scranton MI, Taylor CD, Taylor GT. (2020) Diverse nitrogen cycling pathways across a marine oxygen gradient indicate nitrogen loss coupled to chemoautotrophic activity Environmental Microbiology doi:10.1111/1462-2920.15187
Mara P, Vik D, Pachiadaki MG, Suter EA, Poulos B, Taylor GT, Sullivan MB, Edgcomb VP. (2020). Viral elements and their potential influence on microbial processes along the permanently-stratified Cariaco Basin redoxcline. International Society for Microbial Ecology Journal. https://doi.org/10.1038/s41396-020-00739-3.
Medina Faull L, Mara P, Taylor GT, Edgcomb VP. (2020). Imprint of trace dissolved oxygen on prokaryoplankton community structure in an oxygen minimum zone. Frontiers Mar. Sci. 7:360 doi:10.3389/fmars.2020.00360
Yakubovskaya E, Zaliznyak T, Martinez Martinez J, Taylor GT (2020). Virus purification protocol for Ehv-163 and other viruses. protocols.io dx.doi.org/10.17504/protocols.io.bcpvivn6
Yakubovskaya E, Zaliznyak T, Martinez Martinez J, Taylor GT (2020). New chemiphotobleaching protocol for Raman spectroscopy. protocols.io dx.doi.org/10.17504/protocols.io.bchqit5w
Scranton MI, Taylor GT, Thunell RC, Muller-Karger FE, Astor Y, Swart P, Edgcomb VP, Pachiadaki MG (2020). Anomalous d 13C in POC at the chemoautotrophy maximum in the Cariaco Basin. J Geophys. Res. – Biogeosciences, 125, e2019JG005276; doi :10.1029/2019JG005276.
Yakubovskaya, E, Zaliznyak T, Martínez Martínez J. Taylor GT (2019). Tear down the fluorescent curtain: A new fluorescence suppression method for Raman microspectroscopic analyses. Scientific Reports, 9: 15785. doi:10.1038/s41598-019-52321-3
Taylor GT. (2019). Windows into microbial seascapes: Advances in nanoscale imaging and application to marine science. Annual Review of Marine Sciences 11: 13.1-13.26.
Louca S, Astor YM, Doebeli M, Taylor GT, Scranton MI. (2019) Microbial metabolite fluxes in a model marine anoxic ecosystem. Geobiology 17:628–642. doi: 10.1111/gbi.12357
Weber, F, Zaliznyak, T, Taylor GT (2019) Sample preparation for 3D-Raman microspectroscopic mapping of fully hydrated protist cells. https://www.protocols.io/view/sample-preparation-for-3d-raman-microspectroscopic-ztyf6pw.
Louca S, Scranton MI, Taylor GT, Crowe SA, & Doebeli M. (2019). Circumventing kinetics in biogeochemical models. Proc. Nat’l. Acad. Sci. (USA) https://doi.org/10.1073/pnas.1819883116
Muller-Karger F, Astor Y, Benitez-Nelson C, Buck K, Fanning K, Lorenzoni L, Montes E, Scranton M, Rueda-Roa D, Taylor G, Thunell R, Tappa E, Varela R. (2019). Scientific legacy of the CARIACO oceanographic time-series program. Annual Review of Marine Sciences 11:5.1-5.25.
Jürgens K Taylor GT. (2018) Chap. 7: Microbial ecology and biogeochemistry of oxygen-deficient water columns, In: Microbial Ecology of the Oceans, 3rd Edn., Wiley Blackwell Press (Gasol JM & Kirchman DL, eds.), Chap. 7, p. 231-288.
Taylor GT, Suter EA, Pachiadaki MG, Astor Y, Edgcomb VP, Scranton MI. (2018). Temporal Shifts in dominant sulfur-oxidizing chemoautotrophic populations across the Cariaco Basin’s redoxcline. Deep-Sea Res, https://doi.org/10.1016/j.dsr2.2017.11.016
Suter EA, Pachiadaki MG, Taylor GT, Astor Y, Edgcomb VP. (2018) Free-living chemoautotrophic and particle-attached heterotrophic prokaryotes dominate microbial assemblages along a pelagic marine redox gradient. Environ Microbiol, 20(2): 693-712. doi:10.1111/1462-2920.13997.
Taylor GT, Suter E, Li Z-Q, Chow S, Stinton D, Zaliznyak T, Beaupré S. (2017) Single-cell growth rates in photoautotrophic populations measured by stable isotope probing and resonance Raman microspectrometry. Frontiers in Microbiol doi: 10.3389/fmicb.2017.01449
Cernadas-Martín S, Scranton MI, Astor Y, Taylor GT. (2017) Aerobic and anaerobic ammonia oxidizers in the Cariaco Basin: Identification, quantification and community structure. Aquatic Micrbiol Ecol 79: 31–48, DOI: 10.3354/ame01817.
Kang Y, Tang Y-Z, Taylor GT, Gobler CJ. (2017). Discovery of a resting stage in the harmful, brown tide-causing pelagophyte, Aureoumbra lagunensis: a mechanism facilitating recurring blooms and recent expansion? J Phycol 53: 118-130. DOI: 10.1111/jpy.12485
Suter EA, Scranton MI, Chow S, Medina L, Taylor GT. (2017). Niskin bottle sample collection can alias microbial community composition and biogeochemical interpretation. Limnol Oceanogr 62: 606-617. DOI: 10.1002/lno.10447
Rodriguez-Mora M, Edgcomb V, Taylor C, Taylor GT, Scranton M, Chistoserdov A (2016). The diversity of sulfide oxidation and sulfate reduction genes expressed by the bacterial communities of the Cariaco Basin, Venezuela. Open Microbiol Jour 10: 140-149
Rodriguez-Mora M, Scranton M, Taylor GT, Chistoserdov A. 2015. The dynamics of the bacterial diversity in the redox transition and anoxic zones of the Cariaco Basin assessed by massively parallel tag sequencing. FEMS Microbiology Ecology 91: doi: 10.1093/femsec/fiv088.
Liu L, Lwiza KMM Taylor GT (2015). Importance of the bacterial dynamics in model simulations of seasonal hypoxia. Cont. Shelf Res. 105: 1-17.
Suter E, Lwiza KMM, Rose JM, Gobler C, Taylor GT (2014). Nutrient and phytoplankton regime shifts during decadal decreases in nitrogen loadings to the urbanized Long Island Sound estuary. Mar. Ecol. Prog. Ser. 497: 51-67.
Scranton MI, Taylor GT, Thunell R, Benitez-Nelson C, Muller-Karger F, Fanning K, Lorenzoni L, Montes E, Varela R, Astor Y (2014). Interannual and decadal variability in the nutrient geochemistry of the Cariaco Basin. Oceanography Magazine 27(1): 148-159.
Astor YM, Lorenzoni L, Thunell R, Varela R, Muller-Karger F, Troccoli L , Taylor GT, Scranton MI, Tappa E Rueda D (2013). Interannual variability in sea surface temperature and fCO 2 changes in the Cariaco Basin, Deep-Sea Research II, 93, 33–43.
Sarmento H, Romera-Castillo C, Lindh M, Pinhassi J, Montserrat Sala M, Gasol JM, Marrase´ C, Taylor GT (2013). Phytoplankton species-specific release of dissolved free amino acids and their selective consumption by bacteria. Limnol. Oceanogr. 58(3), 1123–1135.
Orsi W, Edgcomb V, Faria J, Foissner W, Fowle WH, Hohmann T, Suarez P, Taylor C, Taylor GT, Vďačný P & Epstein SS (2012). Class Cariacotrichea, a novel ciliate taxon from the anoxic Cariaco Basin,Venezuela. Internat’l Jour System Evolutionary Microbio. 62: 1425–1433.
Podlaska A, Wakeham SG, Fanning K, Taylor GT (2012). Microbial community structure and chemoautotrophic activity in the oxygen minimum zone of the eastern tropical North Pacific. Deep-Sea Res. I, 66: 77-89.
Taylor GT, Muller-Karger F, Thunell RC, Scranton MI, Astor Y, Varela R, Troccoli-Ghinaglia L, Lorenzoni L, Fanning KA, Hameed S, Doherty O (2012). Ecosystem response to global climate change in the southern Caribbean Sea. Featured Article in Proc. Nat’l. Acad. Sci. (USA) 109(47): 19315-19320.
Finiguerra MB, Escribano DF, Taylor GT (2011). Light-independent mechanisms of virion inactivation in coastal marine systems. Hydrobiologia, 665: 51-66.
Edgcomb V, Orsi W, Taylor GT, Vdacny P, Taylor C, Suarez P, Epstein S (2011). Accessing marine protists from the anoxic Cariaco Basin. J. Int Soc Microb Ecol. 5(8): 1237-1241.
Edgcomb V, Orsi W, Bunge J, Jeon SO, Christen R, Leslin C, Holder M, Taylor GT, Suarez P, Varela R, Epstein S (2011). Protistan microbial observatory in the Cariaco Basin, Caribbean. I. Pyrosequencing vs Sanger insights into species richness. J Int Soc Microb Ecol. 5(8): 1344-1356.
Orsi W, Edgcomb V, Jeon SO, Bunge J, Taylor GT, Varela R, Epstein S (2011). Protistan microbial observatory in the Cariaco Basin, Caribbean. II. Habitat specialization . J Int Soc Microb Ecol. 5(8): 1357-1373.
Taylor GT, Thunell RC, Varela R, Benitez-Nelson C & Scranton MI (2009). Hydrolytic ectoenzyme activity associated with suspended and sinking organic particles above and within the anoxic Cariaco Basin. Deep-Sea Res. I, 56: 1266-1283.
Taylor GT, Scranton MI, Iabichella M, Ho T-Y, Thunell RC, Muller-Karger F, Varela R (2001). Chemoautotrophy in the redox transition zone of the Cariaco Basin: A significant midwater source of organic carbon production. Limnol Oceanogr 46: 148-163.