Nadine Henderson

Research Support Specialist
nadine.henderson@stonybrook.edu

Laboratory of Dr. David Thanassi, 2002 - present

B.S., 1991, University of California, Davis
M.S., 1993, Indiana University

Scottish Crop Research Institute, 1994 - 1996
University of Dundee (Scotland), Department of Anatomy and Physiology, 1996 - 1997
University of Dundee (Scotland), Department of Molecular and Cellular Pathology, 1997 - 2000
Stony Brook University, Department of Pathology, 2000 - 2002

Select Publications:
Innes RW, Bisgrove SR, Smith NM, Bent AF, Staskawicz BJ, and YC Liu. 1993. Identification of a disease resistance locus in Arabidopsis that is functionally homologous to the RPG1 locus of soybean. Plant J. 4:813-820.

Bisgrove SR, Simonich MT, Smith NM, Sattler A, and RW Innes. 1994. A disease resistance gene in Arabidopsis with specificity for two different pathogen avirulence genes. Plant Cell. 6:927-933.

Benos PV, Gatt MK, Ashburner M, Murphy L, Harris D, Barrell B, Ferraz C, Vidal S, Brun C, Demailles J, Cadieu E, Dreano S, Gloux S, Lelaure V, Mottier S, Galibert F, Borkova D, Minana B, Kafatos FC, Louis C, Sidén-Kiamos I, Bolshakov S, Papagiannakis G, Spanos L, Cox S, Madueño E, de Pablos B, Modolell J, Peter A, Schöttler P, Werner M, Mourkioti F, Beinert N, Dowe G, Schäfer U, Jäckle H, Bucheton A, Callister DM, Campbell LA, Darlamitsou A, Henderson NS, McMillan PJ, Salles C, Tait EA, Valenti P, Saunder RD, and DM Glover. 2000. From sequence to chromosome: the tip of the X chromosome of D. melanogaster. Science. 287:2220-2222.

Henderson NS, Nijtmans LG, Lindsay JG, Lamantea E, Zeviani M, and IJ Holt. 2000. Separation of intact pyruvate dehydrogenase complex using blue native agarose gel electrophoresis. Electrophoresis. 21:2925-2931.

Nijtmans LG, Henderson NS, Attardi G, and IJ Holt. 2001. Impaired ATP synthase assembly associated with a mutation in the human ATP synthase subunit 6 gene. J. Biol. Chem. 276:6755-6762.

Benos PV, Gatt MK, Murphy L, Harris D, Barrell B, Ferraz C, Vidal S, Brun C, Demaille J, Cadieu E, Dreano S, Gloux S, Lelaure V, Mottier S, Galibert F, Borkova D, Miñana B, Kafatos FC, Bolshakov S, Sidén-Kiamos I, Papagiannakis G, Spanos L, Louis C, Madueño E, de Pablos B, Modolell J, Peter A, Schöttler P, Werner M, Mourkioti F, Beinert N, Dowe G, Schäfer U, Jäckle H, Bucheton A, Callister D, Campbell L, Henderson NS, McMillan PJ, Salles C, Tait E, Valenti P, Saunders RD, Billaud A, Pachter L, Glover DM, and M Ashburner. 2001. From first base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. Genome Res. 11:710-730.

Peter A, Schöttler P, Werner M, Beinert N, Dowe G, Burkert P, Mourkioti F, Dentzer L, He Y, Deak P, Benos PV, Gatt MK, Murphy L, Harris D, Barrell B, Ferraz C, Vidal S, Brun C, Demaille J, Cadieu E, Dreano S, Gloux S, Lelaure V, Mottier S, Galibert F, Borkova D, Miñana B, Kafatos FC, Bolshakov S, Sidén-Kiamos I, Papagiannakis G, Spanos L, Louis C, Madueño E, de Pablos B, Modolell J, Bucheton A, Callister D, Campbell L, Henderson NS, McMillan PJ, Salles C, Tait E, Valenti P, Saunders RD, Billaud A, Pachter L, Klapper R, Janning W, Glover DM, Ashburner M, Bellen HJ, Jäckle H, and U Schäfer. 2002. Mapping and identification of essential gene functions on the X chromosome of Drosophila. EMBO Rep. 3:34-38.

Nijtmans LG, Henderson NS and IJ Holt. 2002. Blue Native electrophoresis to study mitochondrial and other protein complexes. Methods. 26:327-334.

Henderson NS, So SS, Martin C, Kulkarni R, and DG Thanassi. 2004. Topology of the outer membrane usher PapC determined by site-directed fluorescence labeling.J. Biol. Chem. 279:53747-53754.

Remaut H, Tang C, Henderson NS, Pinkner JS, Wang T, Hultgren SJ, Thanassi DG, Waksman G, and H Li. 2008. Fiber formation across the bacterial outer membrane by the chaperone/usher pathway. Cell. 133:640-652.

Mapingire OS, Henderson NS, Duret G, Thanassi DG, and AH Delcour. 2009. Modulating effects of the plug, helix, and N- and C-terminal domains on channel properties of the PapC usher. J. Biol. Chem. 284:36324-36333.

Henderson NS, Ng TW, Talukder I, and DG Thanassi. 2011. Function of the usher N-terminus in catalysing pilus assembly. Mol. Microbiol. 79:954-967.

Phan G, Remaut H, Wang T, Allen WJ, Pirker KF, Lebedev A, Henderson NS, Geibel S, Volkan E, Yan J, Kunze MB, Pinkner JS, Ford B, Kay CW, Li H, Hultgren SJ, Thanassi DG, and G Waksman. 2011. Crystal structure of the FimD usher bound to its cognate FimC-FimH substrate. Nature. 474:49-53.

Volkan E, Ford BA, Pinkner JS, Dodson KW, Henderson NS, Thanassi DG, Waksman G, and SJ Hultgren. 2012. Domain activities of PapC usher reveal the mechanism of action of an Escherichia coli molecular machine. Proc. Natl. Acad. Sci. U. S. A. 109:9563-9568.

Henderson NS and DG Thanassi. 2013. Purification of the outer membrane usher protein and periplasmic chaperone-subunit complexes from the P and type 1 pilus systems. Methods Mol. Biol. 966:37-52.

Volkan E, Kalas V, Pinkner JS, Dodson KW, Henderson NS, Pham T, Waksman G, Delcour AH, Thanassi DG, and SJ Hultgren. 2013. Molecular basis of usher pore gating in Escherichia coli pilus biogenesis. Proc. Natl. Acad. Sci. U. S. A. 110:20741-20746.

Farabella I, Pham T, Henderson NS, Geibel S, Phan G, Thanassi DG, Delcour AH, Waksman G, and M Topf. 2014. Allosteric signaling in the outer membrane translocation domain of PapC usher. Elife. 3. doi: 10.7554/eLife.03532.

Werneburg GT, Henderson NS, Portnoy EB, Sarowar S, Hultgren SJ, Li H, and DG Thanassi. 2015. The pilus usher controls protein interactions via domain masking and is functional as an oligomer. Nat. Struct. Mol. Biol. 22:540-546.