A 200 ml sample of 0.05% by volume latex (polystyrene) spheres 0.195 um in diameter was created by diluting 1 ml of concentrated (10% by volume) suspension in 200 ml of deionized water. The concentrate was kindly donated by the Duke Scientific Corporation [15].
Using the guidelines explained by Yao etal [14], 16 images were
acquired and combined in the following manner,