When I was desperately browsing through reference websites of 
previous WISE students who had done WISE 187 rotations with Dr. 
Noe, I found a couple of topics of interest. Total Internal 
Reflection Fluroescent Microscope (TIFRM) was one of the 
subjects which was left untouched by previous 
students.Coincidentally I happened to be learning about 
Extracellular Matrix of cells, cellular physiology- as in how 
cells have layers and they can be visualized in various ways. At 
the same time I was in another 187 rotation where depth of the 
slides presented an issue in visualizing cells.These topics 
correlate with the purpose of Total Internal Reflect Fluroescent 
Microscope which is **write here the exact purpose and benefit 
of TIFRM ** Once I realized that there are ways to eliminate the 
depth view- how deep into the slide or cellular content a viewer 
can look into- I was interested in this mechanism. Dr. Noe 
provided me with a link to **enter the website name here for the 
optics/micrscope website** From reading this website I began to 
grasp how complicated it is to construct or even mimick the 
setup of TIRFM. Instead of attempting to perform such a 
particular task of setting up a large scale TIRFM and observe 
the relation of evanescent waves and depth from surface, with 
the help of Dr.Noe I realized the project has to start with 
basic understanding of total internal reflection and eventually 
reach out to other concepts of TIRF.