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upright confocal microscope

Leica TCS SP8 X

Technical Details

  • Upright Leica DM 6000 with adaptive focus, motorized XY-Stage (15 nm step size) and Super Z Galvo (1500µm/ 3 nm step size)
  • Tandem scanner 8 KHz:
    • Non-resonant mode: 1-1800 Hz (3600 Hz bidirectional),7fps for 512x 512 pixels, Max frame size 8192x 8192 pixels
    • Resonant mode: 8000 Hz (16000Hz bidirectional), 28 fps for 512x 512 pixels, Max frame size 1024x 1024 pixels
  • WLL (White Light Laser 470-670 nm), Laser UV 405 nm DMOD flexible
  • Detection range: 400-800 nm
  • Internal detection channels: 2xPMT, 2x HyD
  • Transmitted light detectors: BF (Brightfield)
  • Fluo filters: A (BP 340-380, LP 425), I3 (BP 450-490, LP 515), N2.1 (BP 515-560, LP 590)
  • All objectives have a corresponding DIC (differential interference contrast) condenser prism for DIC measurement in confocal mode
  • Equipped with Tokai Hit Stage Incubator providing 37˚C and 5% CO2 for live cell imaging

confocal image aconfocal image bconfocal image c

Confocal microscope images of (left) Organic photovoltaic polymer MEH-PPV
electrospun fibers;(middle) Dental pulp stem cells grown on polyisoprene
(PI) thin film treated with micro-sized beads taken together by
incident (fluorescence) and transmitted (DIC) lights. Stained with
Alex Flour Phalloidin 488 (actin filaments) and DAPI (nuclei); (right)
Organic photovoltaic polymer P3HT blended with PMMA

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