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Rolf Sternglanz

sternglanzDistinguished Professor Emeritus
Department of Biochemistry and Cell Biology

Life Sciences Building
Stony Brook University
Stony Brook, NY 11794-5215
Office telephone: 631-632-8565
Fax: 631-632-9730


  • Research Description

    Our lab uses the budding yeast, Saccharomyces cerevisiae to identify mutants and characterize genes affecting structure and function of the nucleus. This includes genes encoding proteins involved in gene regulation, DNA replication or chromatin structure. Current effort is focused on: 1) the mechanism of transcriptional silencing of the mating-type loci (i.e., a study of the yeast equivalent of heterochromatin), 2) identification of genes encoding histone acetyltransferases, deacetylases and other histone modifying enzymes, and 3) the role of the conserved KEOPS complex in tRNA modification and other cellular processes.

  • Publication
    1. Vinaya Sampath, Bingsheng Liu, Stefan Tafrov, Madhusudhan Srinivasan, Robert Rieger, Emily I. Chen and Rolf Sternglanz (2013) Biochemical characterization of Hpa2 and Hpa3-two small closely related acetyltransferases from S. cerevisiae. J. Biol. Chem. 288: 21506-21513.
    2. Hsu, H.S., Wang, C.-L., Wang, M., Yang, Z., Chen, Z., Sternglanz, R. and Xu, R.-M. (2013) Structural basis for allosteric stimulation of Sir2 activity by Sir4 binding.Genes & Development. 27: 64-73.
    3. Yuan, H., Rossetto, D., Mellet, H., Dang, W., Srinivasan, M., Hodawadekar, S., Ding, E.C., Speicher, K., Abshiru, N., Perry, R., Speicher, D., Thibault, P., Verreault, A., Berger, S., Sternglanz, R. McMahon, S.B., Côté, J., and Marmorstein, R. (2011) MYST protein acetyltransferase activity requires active site lysine autoacetylation.EMBO J. 31: 58-70.
    4. Yao, Y., Srinivasan, M., Nakanishi, S., Leatherwood, J., Shilatifard, A. and Sternglanz, R. (2011) A conserved patch near the C-terminus of histone H4 is required for genome stability in budding yeast. Mol. Cell. Biol. 31: 2311-2325.
    5. Srinivasan, M., Mehta, P., Yu, Y., Prugar, E., Koonin, E.V., Karzai, A.W. and Sternglanz, R. (2011) The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A. EMBO J. 30: 873-888.
    6. Yu, Y., Neiman, A.M., and Sternglanz, R. (2010) The JmjC domain of Gis1 is dispensable for transcriptional activation. FEMS Yeast Research 10: 793-801.
    7. Ren, J., Wang, C.-L. and Sternglanz, R. (2010) Promoter strength influences the S phase requirement for silencing at the Saccharomyces cerevisiae silent mating type loci.  Genetics 186, 551-560.
    8. Yu, Y., Neiman, A.M., and Sternglanz, R. (2010) The JmjC domain of Gis1 is dispensable for transcriptional activation.  FEMS Yeast Research 10, 793-801.
    9. Sampath, V., Yuan, P., Wang, I, Prugar, E., van Leeuwen, F. and Sternglanz, R. (2009) Mutational analysis of the Sir3 BAH domain reveals multiple points of interaction with nucleosomes.  Mol. Cell.Biol.  29, 2532-25445
    10. Wang, C.-L., Landry, J. and Sternglanz, R. (2008) A yeast Sir2 mutant temperature-sensitive for silencing.  Genetics 180, 1955-1962
    11. Kumar, P.R., Yu, Y., Sternglanz, R., Johnston, S. and Joshua-Tor, L. (2008) NADP regulates the yeast GAL induction system.  Science 319, 1090-1092
    12. Schuetz, A., Min, J., Antoshenko, T., Wang, C.L., Allali-Hassani, A., Dong, A., Loppnau, P. Bochkarev, A., Sternglanz, R. and Plotnikov, A. (2007) Structural basis of inhibition of NAD+-dependent deacetylase SIRT5 by suranim.  Structure 15, 377-389
    13. Krichevsky, A., Gutgarts, H., Kozlovsky, S., Tzfira, T., Sutton, A., Sternglanz, R., Mandel, G. and Citovsky, V. (2007) C2H2 zinc finger-SET histone methyltransferase is a plant-specific chromatin modifier.  Developmental Biology 303, 259-269
    14. Zappulla, D., Maharaj, A., Connelly, J., Jockusch, R. and Sternglanz, R. (2006) Rtt107/Esc4 binds silent chromatin and DNA repair proteins using different BRCT motifs.  BMC Molecular Biology 7, 40.
    15. Vaquero, A., Scher, M., Lee, D., Sutton, A., Cheng, H., Alt, F., Serrano, L, Sternglanz, R. and Reinberg, D. (2006) SirT2 is a histone deacetylase with preference for histone H4 lysine 16 during mitosis. Genes & Development 20: 1256-1261.
    16. Connelly, J., Yuan, P., Hsu, H.-C., Li, Z., Xu, R.-M. and Sternglanz, R. (2006) Structure and function of the Sir3 BAH domain. Mol. Cell. Biol24:3256-3265.
    17. Chu, Y., Sutton, A., Sternglanz, R. and Prelich, G. (2006) The Bur1 cyclin-dependent kinase is required for the normal pattern of histone methylation by Set2. Mol. Cell. Biol. 24: 3029-3038.
    18. Liu, B., Sutton, A. and Sternglanz, R. (2005) A yeast polyamine acetyltransferase. J. Biol. Chem. 280: 16659-16664.
    19. Wang, X., Connelly, J., Wang, C.L. and Sternglanz, R. (2004) Importance the Sir3 N-terminus for yeast transcriptional silencing. Genetics 168: 547-551.
    20. Hess, D., Liu, B., Roan, N., Sternglanz, R. and Winston, F. (2004) Spt10-dependent transcriptional activation in S. cerevisiae requires both the Spt10 acetyltransferase domain and Spt21. Mol. Cell. Biol. 24: 135-143.
    21. Poveda, A., Pamblanco, M., Tafrov, S., Tordera, V., Hsu, H.S., Wang, C.-L., Wang, M., Yang, Z., Chen, Z., Sternglanz, R. and Xu, R.-M. (2013) Structural basis for allosteric stimulation of Sir2 activity by Sir4 binding. Genes & Development. 27: 64-73. and Sendra, R. (2004) Hif1 is a component of yeast histone acetyltransferase complex B, a complex located mainly in the nucleus.J. Biol. Chem. 279: 16033-16043.
    22. Andrulis, E., Zappulla, D., Alexieva-Botcheva, K., Evangelista, C. and Sternglanz, R. (2004) One-hybrid screens at the Saccharomyces cerevisiae HMR locus identify novel transcriptional silencing factors. Genetics 166: 631-635.
    23. Song, O., Wang, X., Waterborg, J. and Sternglanz, R. (2003) An N a-acetyltransferase responsible for acetylation of the N-terminal residues of histones H4 and H2A. J. Biol. Chem. 278: 38109-38112.
    24. Sutton, A., Shia, W., Band, D., Kaufman, P., Osada, S., Workman, J. and Sternglanz, R. (2003) Sas4 and Sas5 are required for the histone acetyltransferase activity of Sas2 in the SAS complex. J. Biol. Chem. 278: 16887-16892.
    25. Landry, J., Sutton, A., Hesman, T., Min, J., Xu, R.-M., Johnston, M. and Sternglanz, R. (2003) Set2-catalyzed methylation of histone H3 represses basal expression of GAL4 in S. cerevisiaeMol. Cell. Biol. 23: 5972-5978.
    26. Andrulis, E., Zappulla, D., Ansari, A., Perrod, S., Laiosa, C., Gartenberg, M. and Sternglanz, R. (2002) Esc1, a nuclear periphery protein required for Sir4-based plasmid anchoring and partitioning. Mol. Cell. Biol. 22: 8292-8301.
    27. Zappulla, D., Sternglanz, R. and Leatherwood, J. (2002) Control of replication timing by a transcriptional silencer. Current Biology 12: 869-875.
    28. Min, J., Landry, J., Sternglanz, R. and Xu, R. (2001) Crystal structure of a SIR2 homolog-NAD complex and proposed mechanism of deacetylation. Cell 105:269-279.
    29. Sutton, A., Heller, R., Landry, J., Choy, J., Sirko, A. and Sternglanz, R. (2001) A novel form of transcriptional silencing by SUM1-1 requires HST1 and the origin recognition complex. Mol. Cell. Biol. 21:3514-3522.
    30. Osada, S., Sutton, A., Muster, N., Brown, C. Yates, J., Sternglanz, R. and Workman, J. (2001) The yeast SAS (something about silencing) protein complex contains a MYST-type acetyltransferase and functions with chromatin assembly factor ASF1. Genes & Devel. 15: 3155-3168.
    31. Landry, J., Sutton, A., Tafrov, S., Heller, R., Stebbins, J., Pillus, L. and Sternglanz, R. (2000) The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases. Proc. Natl. Acad. Sci. 97:5807-5811.
    32. Dutnall, R., Tafrov, S., Sternglanz, R. and Ramakrishnan, V. (1998) Structure of the histone acetyltransferase Hat1: a paradigm for the GCN5-related superfamily.Cell 94: 427-438.
    33. Andrulis, E., Neiman, A., Zappulla, D. and Sternglanz, R. (1998) Perinuclear localization of chromatin facilitates transcriptional silencing. Nature 394: 592-595.
    34. Triolo, T. and Sternglanz, R. (1996) Role of interactions between the origin recognition complex and SIR1 in transcriptional silencing. Nature 381: 251-253.
    35. Chien, C.T., Buck, S., Sternglanz, R. and Shore D. (1993) Targeting of SIR1 protein establishes transcriptional silencing at HM loci and telomeres in yeast. Cell 75: 531-541.
    36. Chien, C.T., Bartel, P.L., Sternglanz, R. and Fields, S. (1991) The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. 88: 9578-9582.