Recent advances in nanotechnology have prompted investigations into nanofibrous scaffolds as vehicles for biomedical applications. Such functions include acting as a medium for gene delivery and a barrier for preventing post-surgical adhesion. To these ends, biodegradable polymer blends such as poly(lactide) (PLA) and poly(lactide-co-glycolide) (PLGA) have been electrospun and extensively studied for their viability in bone and tissue regeneration. By incorporating such biomaterials with principles of gene therapy, progenitor cells may be induced to stimulate tissue regeneration. A scaffold placed in the site of injury provides the mechanical support for cells to proliferate and form a three-dimensional cellular network. Plasmid DNA encoding for biologically active proteins is encapsulated within the scaffold and is released as the scaffold degrades. Progenitor cells in the surrounding tissue migrate towards the degrading scaffold and become transfected by the encapsulated DNA. As transcription and translation occur, the cell differentiates into the target tissue layer. However, a system demonstrating controlled DNA delivery and an efficient transfection rate has yet to be developed. The goal of this research was to engineer a nanofibrous scaffold capable of both long-term release of plasmid DNA and a high transfection efficiency. Scaffolds were electrospun from poly(lactide-co-glycolide) polymers containing plasmid DNA encoding the gene ß-galactosidase. Scaffolds were seeded with MC3T3 pre-osteoblastic mouse cells and incubated at 37 o C. To test whether the cells were indeed transfected and actively translating the protein, X-Gal staining was used to turn the proteins blue. Subsequent Nuclear Fast Red Staining stained the nucleus and cytoplasm of cells red, to ensure that the blue dye was indeed inside a cell. A DNA release assay was then run to determine the degradation profile and percent DNA release of the scaffold.