The purpose of this study was to investigate the drug release pattern from polyurethane (PU) membrane coated with a biodegradable polyelectrolyte complex and an antibiotic -- sulfathiazole. The polyelectrolyte complex consisted of alternating layers of chitosan and hyaluronic acid (HA), as well as polylactic-co-glycolic acid (PLGA). The hypotheses were that higher molecular mass of chitosan and more layers of PLGA would result in slower sulfathiazole release rates for longer durations. In this study, PU membrane was dipped in a medium molecular mass chitosan solution followed by HA/sulfathiazole solution until 10 layers of chitosan and 9 layers of HA/sulfathiazole were coated. The PU membrane was then immersed in phosphate buffered saline and incubated at 37°C. The drug release pattern was recorded and observed until the drug was totally released. Same procedures were repeated 3 times each for medium and high molecular mass chitosan. The results show that the release duration was around 9 hours for both cases, but the higher molecular mass chitosan had a slower average release rate. To test the effect of number of PLGA layers on the drug release pattern, the PU membrane was coated by high molecular mass chitosan and HA in the same sequence mentioned above. The drug release patterns were observed for the three cases of PLGA coated on the outermost layer, on the outermost and half-way point in the chitosan/HA complex, as well as on the outermost, ¼, ½, and ¾ points in the chitosan/HA complex. The release duration were 2 days, 7 days, and 10 days for PU membranes coated with 1, 2 and 4 PLGA layers, respectively. The average release rates tended to be slower as the number of PLGA layers increased. Future studies may include actual clinical testing. This study was supported by grants from the Research and Engineering Apprenticeship Program directed by Prof. Ben Chu, Department of Chemistry at SUNY Stony Brook.