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Fluid
Flow Stimulation of Bone Remodeling Through Intramedullary Pressure Oscillations,
analyzed by Decalcified Bone Embedding.
Kevin Jwo, Mt. Sinai HS, Mt. Sinai, NY; Tamara Kaplan,
Yi-Xian Qin, Department of Biomedical Engineering, Stony Brook University.
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The purpose of this
experiment is to study the effects of fluid flow loading on bone remodeling
in the turkey ulna model. Bone resorptioSn and growth is thought to occur
while being stimulated by intramedullary pressure oscillations. A fluid
loading device was implanted into a hole drilled through the cortex at
the dorsal side of the left ulna of an adult male turkey. Low level, high
frequency oscillations of intramedullary pressure (ImP) were sent through
a saline-filled tube, which connected to the device; this produced the
fluid flow stimulation. The right ulna served as the contra-lateral control,
and was implanted with a nonfunctioning device. Trabecular bone from both
proximal and distal ends were removed through necropsy and processed through
the following steps: decalcifying, dehydrating, clearing, impregnating,
and then embedding in paraffin wax. Sections of approximately 40 microns
were cut with a microtome and then stained with Hematoxylin and Eosin.
After examining the results, no conclusions can be made as to how fluid
loading influenced bone remodeling. This is due in part to the fact that
n = 1. Future studies will include a greater n and will examine in more
detail the effects of fluid loading. This work was funded by the Whitaker
Foundation.
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