Study of Virus-Bacterium Relationships In The Sargasso Sea.
Anish Dhadwal, Ward Melville HS, Setauket NY; and Marina Kutsnova, Cindy Lee, Paul Kemp and Josephine Aller, Marine Sciences Research Center, Stony Brook University.

This experiment examined viral and bacterial concentrations in the Sargasso Sea to assess the possibility of a predator-prey relationship between the two groups of organisms. Within this study, sea-surface microlayer (top 1mm of the water) and subsurface samples were also analyzed to determine the capacity of the nutrient-rich microlayer to support larger populations of bacteria and viral life. Samples were diluted 1:10 using 0.3mL of sample to 2.7mL of distilled water. This dilution effectively lowered the concentration of viral particles per microscope field to workable levels. From here, the samples were passed through a 0.2mm filter, to remove bacterial particles, and vacuum drawn onto a 0.02mm filter. The samples were then dyed with SYBR Gold (2.5x concentration) and counts were done under microscope (using ultraviolet light). Although still in the stages of analyzing data collected, some significant results have been found. In comparing microlayer counts to subsurface counts, the microlayer has been shown to support substantially more bacterial and viral life than subsequent depths of water. These findings bolster claims of the microlayer being an enriched area that ultimately is the basis for all marine food chains. Not enough data has yet been analyzed to determine whether there is a relationship between viruses and bacteria. Ultimately, though, these findings have potential to answer many questions. Red Tides, for example, are known to be massive blooms of bacteria. Red Tides are strange, however, because populations do not simply bloom. Perhaps Red Tides occur when viral populations diminish (as would have in a normal predatory relationship) and bacteria are then left to flourish without considerable predation from bacteria.

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