Science and Engineering Library > Biology Collection > Protocols, Locating Laboratory Procedures
PROTOCOLS, Locating Laboratory Procedures
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An excellent online source for some lab procedures is William Heidcamp's Cell Biology Laboratory Manual - it includes techniques for the Microscope, Histochemistry, Cell Fractionation, Electrophoresis, Enzymes, Membranes, Microsomes, Photosynthesis, Tubules and Filaments, Chromosomes, Cell Cyles and Cultures, Differentiation, and Nucleic Acids. Also provided are guidelines for the representation of statistical data.

Laboratory techniques in cell biology can be found in the classification QH583 - QH585. Plant cell and tissue culture are covered in QK725 while plant molecular biology protocols are in QK728. Guidelines for PCR techniques will be found in QP606. Some of the collection is in oversize, so check the same call numbers there too (always a good idea).

A valuable compilation of cell biology protocols can be found in Cell Biology: A Laboratory Handbook (x QH583.2 .C45). 250 articles cover cell culture, separation, staining, growth, apoptosis, preparation of component parts, assays, antibody production, genetic engineering, microscopy, electrophoresis, and more.

The Essential Data Techniques provide concise protocols in an easy-to-use format. Volumes in the collection are:
  • Cell Biology - QH583.2 .C46
  • DNA isolation and sequencing - QP625.N89 R64
  • Gel electrophoresis : nucleic acids - QP620 .J66
  • Gel electrophoresis : proteins - QP551 .G35
  • PCR - QP606.D46 P34
A companion series Essential Data Series provides protocols and statistical data typically used with these procedures. Volumes are:
  • Animal cells : culture and media - QH585.2 .D37 1994
  • Cell and molecular biology - QH506 .R53 1995
  • Centrifugation - QD54 .C4 R53 1994
  • Enzymes in molecular biology - TP248.E5 E565 1996
  • Gel electrophoresis - QP519.9 .G42 P38 1994
  • Immunoassays - QP519.9.I42 I438 1996
  • Neuropeptides - QP552.N39 H69 1996
  • PCR - QP606.D46 P33 1995
The LabFax series is another set of concise protocols and also include references for further research and information. Volumes are:
  • Biochemistry - QP514.2 .B565 1993
  • Cell Biology - QH585 .C443
  • Cell Culture - QH585 C4 1992
  • Cellular Immunology - QR185.5 .C445 1994
  • Immunochemistry - QR183 .I42 1994
  • Molecular Biology - QH506 .M6624 1998
  • Neuroscience - QP357 .N475 1997
  • Plant Molecular Biology - QK728 .P57 1993
  • Virology - QR201.V55 V55 1993
Finally, the Springer Lab Manuals are another resource:
  • Basic cloning procedures - QH442.2 .B367 1998
  • Basic techniques in molecular biology - QH506 .S89 2000
  • Cell cycle : materials and methods - QH605 .C46
  • Electrotransformation of bacteria - QH448.4 .E43 2000
  • Flow cytometry and cell sorting - QH585.5 .F56 F55
  • Gene transfer to plants - QK981.5 .G45
  • Genetic preservation of plant cells in vitro - QK981.7 .G46
  • In living color : protocols in flow cytometry and cell sorting - QH585.5 .F56 I5 2000
  • Methods in soil biology - QH84.8 .B5713
  • Molecular histochemical techniques - QH452.8 .M65 2000
  • Monoclonal antibodies - QR186.85 .M6813
  • Nonradioactive analysis of biomolecules - QP519.9.A37 N65 2000
  • Nucleic acids in the environment - QP620 .N798
  • Plant cell, tissue, and organ culture : fundamental methods - QK725 .P557
  • Plant molecular biology : a laboratory manual - QK728 .P56
  • Practice of peptide synthesis - QD431.B763 1994
  • Protocols in lichenology - QK583 .P76 2002
  • Quantitation of mRNA polymerase chain reaction - QP623.5.M47 O36
  • Resin microscopy and on-section immunocytochemistry - QR187.I45 N48
More specific and detailed protocols can be found in the Humana Press series: Methods in Molecular Biology. Search STARS by this title to see which individual volumes are available.

A similar series from Academic Press is Methods in Cell Biology. Search STARS by this title to see which individual volumes are available. Cumulative indexes are occasionally published and help provide fast access to the desired protocols.

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Copyright 2003. Last Update: December 2003