ICB&DD Mass Spectrometry Laboratory, Lab 576, Chemistry Bldg.

BRUKER AUTOFLEXII, MALDI-TOF/TOF

lab photoTO RESERVE INSTRUMENT TIME 

The Bruker AutoFlexII instrument provides MALDI-TOF and TOF/TOF (LIFT™) technology for reliable, detailed protein/peptide characterization and lipid, glycan, oligonucleotide and polymer analysis. The design of the instrument allows data acquisition such as linear mode for screening applications, reflectron mode for identification and MS/MS mode (TOF/TOF) for characterization.

The purpose of the laboratory is to provide mass spectral expertise to members of the ICB&DD and external users. Samples may be submitted for analysis and/or approved users may be trained and qualified to operate the instrument. For further information, please contact Dr. Béla Ruzsicska (bela.ruzsicska@stonybrook.edu).

Top Down Sequencing

of Intact Proteins

MALDI-TDS (Top Down Sequencing) is a method for characterization of purified proteins without prior digestion. The In-Source-Decay (ISD) fragmentation occurs rapidly during the MALDI process in the plume, and provides long, contiguous c- and z+2-ion sequence tags accompanied by a- and y-ions. This enables extended N- and C-terminal protein sequences to be derived from single spectra.

 

ICB&DD * 717 Chemistry Bldg., Stony Brook University, Stony Brook, NY 11794-3400
Phone: 631.632.1311 * Fax: 631.632.7942  Email: roxanne.brockner@stonybrook.edu 

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