ICB&DD Mass Spectrometry Laboratory, Lab 576, Chemistry Bldg.
BRUKER AUTOFLEXII, MALDI-TOF/TOF
The Bruker AutoFlexII instrument provides MALDI-TOF and TOF/TOF (LIFT™) technology for reliable, detailed protein/peptide characterization and lipid, glycan, oligonucleotide and polymer analysis. The design of the instrument allows data acquisition such as linear mode for screening applications, reflectron mode for identification and MS/MS mode (TOF/TOF) for characterization.
The purpose of the laboratory is to provide mass spectral expertise to members of the ICB&DD and external users. Samples may be submitted for analysis and/or approved users may be trained and qualified to operate the instrument. For further information, please contact Dr. Béla Ruzsicska (email@example.com).
Top Down Sequencing
of Intact Proteins
MALDI-TDS (Top Down Sequencing) is a method for characterization of purified proteins without prior digestion. The In-Source-Decay (ISD) fragmentation occurs rapidly during the MALDI process in the plume, and provides long, contiguous c- and z+2-ion sequence tags accompanied by a- and y-ions. This enables extended N- and C-terminal protein sequences to be derived from single spectra.
Online Abstract System
Biochemistry & Cell Biology
Physiology & Biophysics
Molecular Genetics & Microbiology
Applied Mathematics and Statistics
Oral Biology and Pathology
Phone: 631.632.1311 * Fax: 631.632.7942 Email: firstname.lastname@example.org