PhD Student in the Department of Molecular Genetics and Microbiology
‘A Poliovirus Cold Case: A Mutation in the Vicinity of a Capsid Interacting
Synopsis: Poliovirus (PV) is a plus strand RNA virus that causes poliomyelitis. PV belongs to the Picornaviridae family and in the genus Enterovirus. PV 2CATPase is a complex polypeptide with important roles in RNA replication, and encapsidation. Two triple alanine mutations upstream of a structural protein interacting sites yielded a replication defective and a lethal growth phenotype. The lethal mutations mapped to a secondary structure in a Phyre2 predicted 2CATPase 3D model. Further investigation into lethal mutations revealed a quasi-infections mutant and a cold-sensitive growth phenotype mutant that arises from viral encapsidation.
Biography: Emmanuel Asare was born in Ghana and migrated to the Bronx, NY. He graduated from Clarkson University with a BS in Biology. In his undergraduate summers, he participated in REU programs at Fordham University, Clarkson University (McNair) and the American Museum of Natural History. He received the NSF BD Fellowship to Stony Brook University’s Genetics program and is currently an AGEP-T FRAME Fellow. Emmanuel is mentored by Dr. Eckard Wimmer and Dr. Aniko Paul at the Molecular Genetics and Microbiology Department.