William J. Lennarz, Ph.D.
Department of Biochemistry and Cell Biology
Institute for Cell and Developmental Biology
Life Sciences Building
Stony Brook University
Stony Brook, NY 11794-5215
Biosynthesis and catabolism of glycoproteins
Our laboratory is interested in understanding several steps involved in glycoprotein synthesis, including N-glycosylation and protein folding, as well as the functions of the glycan chains. We are using yeast, a simple eukaryotic organism that can be genetically manipulated, to study glycoprotein assembly. More specifically, we are investigating the enzymatic processes of oligosaccharide addition and removal that occur in nascent polypeptides and misfolded glycoproteins, respectively ( fig.1 ). Evidence has been obtained that the enzyme PNGase associates with the proteasome during proteolysis of glycoproteins. In addition, PDI, the enzyme that catalyzes folding and disulfide bond formation in glycoproteins, is being studied ( fig.2 ). The crystal structure has been obtained and now we are studying the role of the various domains of this protein in its catalytic activity.
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