Selected Publications Since 2000

1. Citovsky, V. & Zambryski, P. (2000) Systemic transport of RNA in plants. Trends Plant Sci. 5, 52-54. [pdf reprint]
2. Rhee, Y., Tzfira, T., Chen, M.H., Waigmann, E., & Citovsky, V. (2000) Cell-to-cell movement of tobacco mosaic virus: enigmas and explanations. Mol. Plant Pathol. 1, 33-39. [pdf reprint]
3. Tzfira, T., Rhee, Y., Chen, M.H., Kunik, T., & Citovsky, V. (2000) Nucleic acid transport in plant-microbe interactions: the molecules that walk through the walls. Annu. Rev. Microbiol. 54, 187-219. [pdf reprint]
4. Tzfira, T. & Citovsky, V. (2000) From host recognition to T-DNA integration: the function of bacterial and plant genes in the Agrobacterium-plant cell interaction. Mol. Plant Pathol. 1, 201-212. [pdf reprint]
5. Rhee, Y., Gurel, F., Gafni, Y., Dingwall, C., & Citovsky, V. (2000) A genetic system for detection of protein nuclear import and export. Nat. Biotechnol. 18, 433-437. [pdf reprint]
6. Chen, M.H., Sheng, J., Hind, G., Handa, A., & Citovsky, V. (2000) Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell-to-cell movement. EMBO J. 19, 913-920. [pdf reprint]
7. Waigmann, E., Chen, M.H., Bachmaier, R., Ghoshroy, S., & Citovsky, V. (2000) Regulation of plasmodesmal transport by phosphorylation of tobacco mosaic virus cell-to-cell movement protein. EMBO J. 19, 4875-4884. [pdf reprint]
8. Kunik, T., Tzfira, T., Kapulnik, Y., Gafni, Y., Dingwall, C., & Citovsky, V. (2001) Genetic transformation of HeLa cells by Agrobacterium. Proc. Natl. Acad. Sci. USA  98, 1871-1876. [pdf reprint]
9. Ueki, S. & Citovsky, V. (2001) Intercellular RNA transport in plants. Curr. Top.  Plant Biol. 2, 167-179.
10. Tzfira, T. & Citovsky, V. (2001) Comparison between nuclear import of nopaline- and octopine-specific VirE2 protein of Agrobacterium in plant and animal cells. Mol. Plant Pathol. 2, 171-176. [pdf reprint]
11. Tzfira, T., Vaidya, M., & Citovsky, V. (2001) VIP1, an Arabidopsis protein that interacts with Agrobacterium VirE2, is involved in VirE2 nuclear import and Agrobacterium infectivity. EMBOJ. 20, 3596-3607 . [pdf reprint]
12. Ueki, S. & Citovsky, V. (2001) Inhibition of post transcriptional gene silencing by non-toxic concentrations of cadmium. Plant J. 28, 283-291. [pdf reprint]
13. Ueki, S. & Citovsky, V. (2001) RNA commutes to work: regulation of plant gene expression by systemically transported RNA molecules. BioEssays 23, 1087-1090. [pdf reprint]
14. Tzfira, T. & Citovsky, V. (2002) Partners-in-infection: host proteins involved in genetic transformation of plant cells by Agrobacterium. Trends Cell Biol. 12, 121-129. [pdf reprint]
15. Ueki, S. & Citovsky, V. (2002) The systemic movement of a tobamovirus is inhibited by a cadmium-ion-induced glycine-rich protein. Nat. Cell Biol. 4, 478-485. Cover Article [pdf reprint]
16. Tzfira, T., Vaidya, M., & Citovsky, V. (2002) Increasing plant susceptibility to Agrobacterium infection by overexpression of the Arabidopsis VIP1 gene. Proc. Natl. Acad. Sci. USA  99, 10435-10440. [pdf reprint]
17. Zhu, Y., Nam, J., Humara, J.M., Mysore, K., Lee, L.Y., Cao, H., Valentine, L., Li, J., Kaiser, A., Kopecky, A., Hwang, H.H., Bhattacharjee, S., Rao, P., Tzfira, T., Rajagopal, J., Yi, H.C., Yadav, V.B.S., Crane, Y., Lin, K., Larcher, Y., Gelvin, M., Knue, M., Zhao, X., Davis, S., Kim, S.I., Kumar, C.T.R., Choi, Y.J., Hallan, V., Chattopadhyay, S., Sui, X., Ziemienowitz, A., Matthysse, A.G., Citovsky, V., Hohn, B., & Gelvin, S.B. (2003) Identification of Arabidopsis rat mutants. Plant Physiol. 132, 494-505. [pdf reprint]
18. Wu, X., Dinneny, J.R., Crawford, K.M., Rhee, Y., Citovsky, V., Zambryski, P.C., & Weigel, D. (2003) Models of intercellular transcription factor movement in the Arabidopsis apex. Development 130, 3735-3745. [pdf reprint]
19. Chen, M.H. & Citovsky, V. (2003) Systemic movement of a tobamovirus requires host cell pectin methylesterase. Plant J. 35, 386-392. Cover Article [pdf reprint]
20. Tzfira, T. & Citovsky, V. (2003) The Agrobacterium-plant cell interaction: taking biology lessons from a bug. Plant Physiol. 133, 943-947. [pdf reprint]
21. Tzfira, T., Frankman, L., Vaidya, M., & Citovsky, V. (2003) Site-specific integration of Agrobacterium T-DNA via double-stranded intermediates. Plant Physiol. 133, 1011-1023. Cover Article [pdf reprint]
22. Avivi, y., morad, v., ben- meir, h., zhao, j., kashkush, k., Tzfira, t., Citovsky, V., & Grafi, G. (2004) Reorganization of specific chromosomal domains and activation of silent genes in plant cells acquiring pluripotentiality. Dev. Dyn. 230, 12-22. [pdf reprint]
23. Abu-Arish, A., Frenkiel-Krispin, D., Fricke, T., Tzfira, T., Citovsky, V., Grayer Wolf, S., & Elbaum, M. (2004) Three-dimensional reconstruction of Agrobacterium VirE2 protein with single-stranded DNA. J. Biol. Chem. 279, 25359-25363. [pdf reprint]
24. Eulálio, A., Nunes-Correia, I., Carvalho, A.L., Faro, C., Citovsky, V., Simões, S., & Pedroso de Lima, M.C. (2004) Two African swine fever virus proteins derived from a common precursor exhibit different nucleocytoplasmic transport activities. J. Virol. 78, 9731-9739. [pdf reprint]
25. Wang, Y., Tzfira, T., Gaba, V., Citovsky, V., Palukaitis, P. & Gal-On, A. (2004) Functional analysis of the cucumber mosaic virus 2b protein: pathogenicity and nuclear localization. J. Gen. Virol. 85, 3135-3147. [pdf reprint]
26. Waigmann, E., Ueki, S., Trutnyeva, K., & Citovsky, V. (2004) The ins and outs of non-destructive cell-to-cell and systemic movement of plant viruses. Crit. Rev. Plant Sci. 23, 195-250. [pdf reprint]
27. Tzfira, T., Li, J., Lacroix, B., & Citovsky, V. (2004) Agrobacterium T-DNA integration: molecules and models. Trends Genet. 20, 375-383. [pdf reprint]
28. Citovsky , V., Kapelnikov , A., Oliel , S., Zakai, N., Rojas , M.R., Gilbertson , R.L., Tzfira , T., & Loyter , A. (2004) Protein interactions involved in nuclear import of the Agrobacterium VirE2 protein in vivo and in vitro. J. Biol. Chem. 279, 29528-29533. [pdf reprint]
29. Tian, G.W., Mohanty, A., Chary, S.N., Li, S., Paap, B., Drakakaki, G., Kopec, C.D., Li, J., Ehrhardt, D., Jackson, D., Rhee, S.Y., Raikhel, N.V., & Citovsky, V. (2004) High-throughput fluorescent tagging of full-length Arabidopsis gene products in planta. Plant Physiol. 135, 25-38. Cover Article [pdf reprint]
30. Tzfira, T., Vaidya, M., & Citovsky, V. (2004) Involvement of targeted proteolysis in plant genetic transformation by Agrobacterium. Nature 431, 87-92. [pdf reprint]
31. Nuclear Import and Export in Plants and Animals (2005) (T. Tzfira & V. Citovsky, eds.) Eurekah.com and Kluwer Acad./Plenum Publ., 223 pages. Cover. [pdf reprint]
32. Loyter, A., Rosenbluh, J., Zakai, N., Li, J., Kozlovsky, S.V., Tzfira, T., & Citovsky, V. (2005) The plant VirE2 interacting protein 1 . A molecular link between the Agrobacterium T-complex and the host cell chromatin? Plant Physiol. 138, 1318-1321. [pdf reprint]
33. Tzfira, T., Tian, G.W., Lacroix, B., Vyas, S., Li, J., Leitner-Dagan, Y., Krichevsky, A., Taylor, T., Vainstein, A., & Citovsky, V. (2005) pSAT vectors: a modular series of plasmids for fluorescent protein tagging and expression of multiple genes in plants. Plant Mol. Biol. 57, 503-516. Cover Article [pdf reprint]
34. Nelson, R.S. & Citovsky, V. (2005) Plant viruses: invaders of cells and pirates of cellular pathways. Plant Physiol. 138, 1809-1814. [pdf reprint]
35. Chen, M.H., Tian, G.W., Gafni, Y., & Citovsky, V. (2005) Effects of calreticulin on viral cell-to-cell movement. Plant Physiol. 138, 1866-1876. Cover Article [pdf reprint]
36. Lacroix, B., Tzfira, T., Vaidya, M., & Citovsky, V. (2005) The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformation. EMBO J. 24, 428-437. [pdf reprint]
37. Ueki, S., & Citovsky, V. (2005) Control improves with age: systemic transport in plant embryos and adults. Proc. Natl. Acad. Sci. USA 102, 1817-1818. [pdf reprint]
38. Li, J., Vaidya, M., White, C., Vainstein, A., Citovsky, V., & Tzfira, T. (2005) Involvement of KU80 in T-DNA integration in plant cells. Proc. Natl. Acad. Sci. USA 102, 19231-19236. [pdf reprint]
39. Li, J., Krichevsky, A., Vaidya, M., Tzfira, T., & Citovsky, V. (2005) Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by Agrobacterium. Proc. Natl. Acad. Sci. USA 102, 5733-5738. [pdf reprint]
40. Ueki, S. & Citovsky, V. (2005) Identification of an interactor of cadmium ion-induced glycine-rich protein involved in regulation of callose levels in plant vasculature. Proc. Natl. Acad. Sci. USA 102, 12089-12094. [pdf reprint]
41. Eulálio, A., Nunes-Correia, I., Carvalho, A.L., Faro, C., Citovsky, V., Salas, J., Salas, M.L., Simões, S., & Pedroso de Lima, M.C. (2006) Nuclear export of African swine fever virus p37 protein occurs through two distinct pathways and is mediated by three independent signals. J. Virol. 80, 1393-1404. [pdf reprint]
42. Tzfira, T., Kunik, T., Gafni, Y., & Citovsky, V. (2006) Agrobacterium protocols: mammalian cells. Methods Mol Biol. 344, 435-451. [pdf reprint]
43. Tzfira, T. & Citovsky, V. (2006) Agrobacterium-mediated genetic transformation of plants: biology and biotechnology. Curr. Opin. Biotechnol. 17, 147-154. [pdf reprint]
44. Lacroix, B., Li, J., Tzfira, T., & Citovsky, V. (2006) Will you let me use your nucleus? How Agrobacterium gets its T-DNA expressed in the host plant cell. Can. J. Physiol. Pharmacol. 84, 333-345. [pdf reprint]
45. Krichevsky, A., Kozlovsky, S.V., Gafni, Y., & Citovsky, V. (2006) Nuclear import and export of plant viral proteins and genomes. Mol. Plant Pathol. 7, 131-146. Cover Article [pdf reprint]
46. Lacroix, B., Tzfira, T., Vainstein, A., & Citovsky, V. (2006) A case of promiscuity: Agrobacterium’s endless hunt for new partners. Trends Genet. 22, 29-37. Cover Article. [pdf reprint]
47. Tian, G.W., Chen, M.H., Zaltsman, A., & Citovsky, V. (2006) A pollen-specific pectin methylesterase involved in pollen tube growth. Dev. Biol. 294, 83-91. [pdf reprint]
48. Ueki, S. & Citovsky, V. (2007) Spread throughout the plant: systemic transport of viruses. Plant Cell Monogr. 7, 85-118.
49. Citovsky, V., Lee, L.Y., Vyas, S., Glick, E., Chen, M.H., Vainstein, A., Gafni, Y., Gelvin, S.B., & Tzfira, T. (2006) Identification of subcellular localization of interacting proteins by bimolecular fluorescence complementation in planta. J. Mol. Biol. 362, 1120-1131. [pdf reprint]
50. Chakrabarty, R., Banerjee, R., Chung, S-M., Farman, M., Citovsky, V., Hogenhout, S. A., Tzfira, T., & Goodin, M. (2007) pSITE vectors for stable integration or transient expression of autofluorescent protein fusions in plants: probing Nicotiana benthamiana-virus interactions. Mol. Plant-Microbe Interact. 20, 740-750. [pdf reprint]
51. Sandal, I., Saini, U., Lacroix, B., Bhattacharya, A., Ahuja, P.S., & Citovsky, V. (2007) Agrobacterium-mediated genetic transformation of tea leaf explants: effects of counteracting bactericidity of leaf polyphenols without loss of bacterial virulence. Plant Cell Rep. 26, 169-176. [pdf reprint]
52. Zrachya, A., Glick, E., Levy, A., Arazi, T., Citovsky, V., & Gafni, Y. (2007) Suppressor of RNA silencing encoded by Tomato yellow leaf curl virus-Israel. Virology 358, 159-165. Cover Article. [pdf reprint]
53. Anand, A., Krichevsky, A., Schornack, S., Lahaye, T., Tzfira, T., Citovsky, V., & Mysore, K.S. (2007) Arabidopsis VIRE2 INTERACTING PROTEIN2 is required for Agrobacterium T-DNA integration in plants.. Plant Cell 19, 1695-1708. [pdf reprint]
54. Citovsky, V., Kozlovsky, S.V., Lacroix, B., Zaltsman, A., Dafni, M., Vyas, S., Tovkach, A., & Tzfira, T. (2007) Biological systems of the host cell involved in Agrobacterium infection. Cell. Microbiol. 9, 9-20. [pdf reprint]
55. Krichevsky, A., Kozlovsky, S.V., Tian, G.W., Chen, M.H., Zaltsman, A., & Citovsky, V. (2007) How pollen tubes grow. Dev. Biol. 303, 405-420. [pdf reprint]
56. Krichevsky, A., Gutgarts, H., Kozlovsky, S.V., Tzfira, T., Sutton, A., Sternglanz, R., Mandel, G., & Citovsky, V. (2007) C2H2 zinc finger-SET histone methyltransferase, is a plant-specific chromatin modifier. Dev. Biol. 303, 259-269. [pdf reprint]
57. Krichevsky, A., Kozlovsky, S.V., Gutgarts, H., & Citovsky, V. (2007) Arabidopsis co-repressor complexes containing amino oxidase-like proteins and plant-specific histone methyltransferases. Plant Signal. Behav. 2, 174-177. [pdf reprint]
58. Zaltsman, A., Yi, B.Y., Gafni, Y., & Citovsky, V. (2007) Yeast-plant coupled vector system for identification of nuclear proteins. Plant Physiol. 145, 1264-1271. Cover Article. [pdf reprint]
59. Tzfira, T., Kozlovsky, S.V., & Citovsky, V. (2007) Advanced expression vector systems: new weapons for plant research and biotechnology. Plant Physiol. 145, 1087-1089. [pdf reprint]
60. Agrobacterium (2008) (T. Tzfira & V. Citovsky, eds.) Springer, 735 pages. Cover. [pdf reprint]
61. Glick, E., Zrachya, A., Levy, Y., Mett, A., Gidoni, D., Belausov, E., Citovsky, V., & Gafni, Y. (2008) Interaction with the host SGS3 is required for suppression of RNA silencing by the Tomato yellow leaf curl virus V2 protein. Proc. Natl. Acad. Sci. USA 105, 157-161. [pdf reprint]
62. Lacroix, B., Kozlovsky, S.V., & Citovsky, V. (2008) Recent patents on Agrobacterium-mediated gene and protein transfer, for research and biotechnology. Recent Pat. Gene DNA Seq. 2, 69-81. [pdf reprint]
63. Tzfira, T. & Citovsky, V. (2008) Probing interactions between plant virus movement proteins and nucleic acids. Methods Mol. Biol. 451, 293-316. [pdf reprint]
64. Petrunia, I.V., Frolova, O., Komarova, T.V., Kiselev, S.L., Citovsky, V., & Dorokhov, Y. (2008) Agrobacterium tumefaciens-induced bacteraemia does not lead to reporter gene expression in mouse organs. PLoS ONE 3, e2352. [pdf reprint]
65. Citovsky, V., Gafni, Y., & Tzfira, T. (2008) Localizing protein-protein interactions by bimolecular fluorescence complementation in planta. Methods 45, 196-206. [pdf reprint]
66. Lacroix, B., Loyter, A., & Citovsky, V. (2008) Association of the Agrobacterium T-DNA-protein complex with plant nucleosomes. Proc. Natl. Acad. Sci. USA 105, 15429-15434. [pdf reprint]
67. Orlova, I., Nagegowdaa, D.A., Kish, C.M., Gutensohn, M., Maeda, H. Varbanova, M., Fridman, E., Yamaguchi, S., Hanada, S., Kamiya, Y., Krichevsky, A., Citovsky, V., Pichersky, E., & Dudareva, N. (2009) Small subunit of geranyl diphosphate synthase modifies the chain length specificity of phylogenetically distant geranylgeranyl diphosphate synthase in planta. Plant Cell 21, 4002-4017. [pdf reprint]
68. Krichevsky, A., Zaltsman, A., Kozlovsky, S.V., Tian, G.W., & Citovsky, V. (2009) Regulation of root elongation by histone acetylation in Arabidopsis. J. Mol. Biol. 385, 45-50. [pdf reprint]
69. Lacroix, B. & Citovsky, V. (2009) Agrobacterium aiming for the host chromatin: host and bacterial proteins involved in interactions between T-DNA and plant nucleosomes. Communic. Integr. Biol. 2, 42-45. [pdf reprint]
70. Tzfira, T. & Citovsky, V. (2009) Systems biology of plant-pathogen interactions. Semin. Cell Dev. Biol. 20, 1015-1016. [pdf reprint]
71. Citovsky, V., Zaltsman, A., Kozlovsky, S.V., Gafni, Y., & Krichevsky, A. (2009) Proteasomal degradation in plant-pathogen interactions. Semin. Cell Dev. Biol. 20, 1048-1054. [pdf reprint]
72. Ueki, S., Lacroix, B., Krichevsky, A., Lazarowitz, S.G., & Citovsky, V. (2009) Functional transient transformation of intact Arabidopsis plants by microbombardment. Nat. Protoc. 4, 71-77. [pdf reprint]
73. Komarova, T.V., Schwartz, A.M., Frolova, O.Y., Zvereva, A.S., Gleba, Y.Y., Citovsky, V. & Dorokhov, Y.L. (2010) Pol II-directed non-coding RNAs suppress nuclear export of mRNA. Plant Mol. Biol. 74, 591-603.
74. Zaltsman, A., Kozlovsky, S.V., Yasmin, F., & Citovsky, V. (2010) Plant defense pathways subverted by Agrobacterium for genetic transformation. Plant Signal. Behav. 5, 1245-1248. [pdf reprint]
75. Ueki, S., Meyers, B.L., Yasmin, F., & Citovsky, V. (2010) A cell-to-cell macromolecular transport assay in planta utilizing biolistic bombardment. J. Vis. Exp. 42, pii 2208. [pdf reprint]
76. Bhattacharya, A., Sood, P., & Citovsky, V. (2010). The roles of plant phenolics in defense and communication during Agrobacterium and Rhizobium infection. Mol. Plant Pathol. 11, 705-719. [pdf reprint]
77. Ueki, S., Spektor, R., Natale, D.M., & Citovsky, V. (2010) ANK, a host cytoplasmic receptor for the Tobacco mosaic virus cell-to-cell movement protein, down-regulates callose levels at plasmodesmata to control intercellular transport. PLoS Pathog. 6, e1001201. [pdf reprint]
78. Krichevsky, A., Meyers, B.L., Vainstein, A., Maliga, P., & Citovsky, V. (2010) Autoluminescent plants. PLoS ONE 5, e15461. [pdf reprint]
79. Zaltsman, A., Krichevsky, A., Loyter, A., & Citovsky, V. (2010) Agrobacterium induces expression of a host F-box protein required for tumorigenicity. Cell Host Microbe 7, 197-209. [pdf reprint]
80. Plant Transformation Technologies (2011) (N. Stewart, Jr., A. Touraev, V. Citovsky, & T. Tzfira, eds.) Wiley-Blackwell, 296 pages.
81. Ueki, S., Magori, S., Lacroix, B., & Citovsky, V. (2011) Transient gene expression in plants by biolistic DNA delivery. Methods Mol. Biol., in press.
82. Magori, S. & Citovsky, V. (2011) Hijacking of the host SCF ubiquitin ligase machinery by plant pathogens. Front. Plant Sci., in press.
83. Zavaliev, R., Ueki, S., Epel, B.L., & Citovsky, V. (2011) Biology of callose (ß-1,3-glucan) turnover at plasmodesmata. Protoplasma 248, 117-130 [pdf reprint].
84. Ueki, S. & Citovsky, V. (2011) Protein membrane overlay assay: a protocol to test interaction between soluble and insoluble proteins in vitro. J. Vis. Exp. 54, pii 2961. [pdf reprint]
85. Magori, S. & Citovsky, V. (2011) Epigenetic control of Agrobacterium T-DNA integration. Biochim. Biophys. Acta 1809, 388-394. [pdf reprint]
    
86. Ueki, S. & Citovsky, V. (2011) To gate, or not to gate: regulatory mechanisms for intercellular protein transport and virus movement in plants. Mol. Plant 4, 782-793. [pdf reprint]
87. Lacroix, B. & Citovsky, V. (2011) Extracellular VirB5 enhances T-DNA transfer from Agrobacterium to the host plant. PLoS ONE 6, e25578. [pdf reprint]
88. Krichevsky, A., Zaltsman, A., Lacroix, B., & Citovsky, V. (2011) Involvement of KDM1C histone demethylase-OTLD1 otubain-like histone deubiquitinase complexes in plant gene repression. Proc. Natl. Acad. Sci. USA 108, 11157-11162. [pdf reprint]
89. Magori, S. & Citovsky, V. (2011) Agrobacterium counteracts host-induced degradation of its F-box protein effector. Sci. Signal. 4, ra69. Cover Article. [pdf reprint]
90. Lacroix, B. & Citovsky, V. (2012) Agrobacterium. In Brenner’s Online Encyclopedia of Genetics Second Edition (S. Maloy & K. Hughes, eds.), Elsevier, vol. 1, in press. [pdf reprint]
91. Lacroix, B. & Citovsky, V. (2012) Crown Gall Tumors. In Brenner’s Online Encyclopedia of Genetics Second Edition (S. Maloy & K. Hughes, eds.), Elsevier, vol. 1, in press.

Patents

1. Citovsky, V. (1998) “Protein-Mediated Nuclear Import of DNA”. U.S. patent # 5,831,020. [pdf reprint]
2. Rhee, Y. & Citovsky, V. (2005) “Genetic Assay for Protein Nuclear Transport”. U.S. patent # 6,902,886. [pdf reprint]
   
3. Tzfira, T. & Citovsky, V. (2010) “Increasing Host Plant Susceptibility to Agrobacterium Infection by Overexpression of the Arabidopsis VIP1 Gene”. U.S. patent # 7,659,447. [pdf reprint]

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