Distinguished Professor
Department of Biochemistry and Cell Biology
SUNY at Stony Brook
Ph.D., Harvard University, 1967
Yeast molecular genetics
Our lab uses the budding yeast, Saccharomyces cerevisiae to identify mutants and characterize genes affecting structure and function of the nucleus. This includes genes encoding proteins involved in gene regulation, DNA replication or chromatin structure. Current effort is focused on: 1) the mechanism of transcriptional silencing of the mating-type loci (i.e., a study of the yeast equivalent of heterochromatin), 2) identification of mutants and genes involved in histone acetylation and deacetylation, 3) the role of the nuclear periphery in regulation of DNA replication and silencing.
Selected References:
Triolo, T. and Sternglanz, R. (1996) Role of interactions between the origin recognition complex and SIR1 in transcriptional silencing. Nature 381, 251-253.
Andrulis, E., Neiman, A., Zappulla, D. and Sternglanz, R. (1998) Perinuclear localization of chromatin facilitates transcriptional silencing. Nature 394, 592-595.
Dutnall, R., Tafrov, S., Sternglanz, R. and Ramakrishnan, V. (1998) Structure of the histone acetyltransferase Hat1: a paradigm for the GCN5-related N-acetyltransferase superfamily. Cell 94, 427-438.
Sternglanz, R. and Schindelin, H. (1999) Commentary. Structure and mechanism of action of the histone acetyltransferase Gcn5 and similarity to other N-acetyltransferases. Proc. Natl. Acad. Sci. USA 96, 8807-8808.
John, S., Grant, P., Tafrov, S., Sternglanz, R. and Workman, J. (2000) The Something About Silencing protein, SAS3, is the catalytic subunit of NuA3, a TAF30-containing HAT complex that interacts with the SPT16 subunit of the yeast CP (Cdc68/Pob3) FACT complex. Genes & Develop. 14, 1196-1208.
Landry, J., Sutton, A., Tafrov, S., Heller, R., Stebbins, J., Pillus, L. and Sternglanz, R. (2000) The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases. Proc. Natl. Acad. Sci. 97, 5807-5811.
Tanner, K., Landry, J., Sternglanz, R. and Denu, J. (2000) SIR2 family of NAD-dependent histone/protein deacetylases genererates a unique product, 1-O-acetyl-ADP-ribose. Proc. Natl. Acad. Sci. 97, 14178-14182.
Landry, J., Slama, J. and Sternglanz, R. (2000) Role of NAD+ in the deacetylase activity of the SIR2-like proteins. Biochem. and Biophys. Res. Comm. 278, 685-690.
Sutton, A., Heller, R.,
Landry, J., Choy, J., Sirko, A. and Sternglanz, R. (2001) A novel form of transcriptional
silencing by SUM1-1 requires HST1 and the origin recognition complex. Mol.
Cell. Biol. 21, 3514-3522.
Sutton, A., Bucaria, J., Osley, M.A. and Sternglanz, R. (2001) Yeast ASF1 proteins
is required for cell-cycle regulation of histone gene transcription. Genetics
158, 587-596.
Min, J., Landry, J., Sternglanz, R. and Xu, R. (2001) Crystal structure of a
SIR2 homolog-NAD complex and proposed mechanism of deacetylation. Cell 105,
269-279.
Osada, S., Sutton, A., Muster, N., Brown, C. Yates, J., Sternglanz, R. and Workman,
J. (2001) The yeast SAS (something about silencing) protein complex contains
a MYST-type acetyltransferase and functions with chromatin assembly factor ASF1.
Genes & Devel. 15, 3155-3168.
Zappulla, D., Sternglanz, R. and Leatherwood, J. (2002) Control of replication timing by a transcriptional silencer. Current Biology 12, 869-875.
Andrulis, E., Zappulla, D., Ansari, A., Perrod, S., Laiosa, C., Gartenberg, M. and Sternglanz, R. (2002) Esc1, a nuclear periphery protein required for Sir4-based plasmid anchoring and partitioning. Mol. Cell. Biol. 22, 8292-8301.