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PlastBHA3 forms normal filaments in SW13c1.1Vim+ cells (A), but is unable to self-assemble in the absence of vimentin in SW13c1.2Vim- cells (B). A dual labeling immunofluorescence assays shows that plastBHA3 (C) colocalizes with vimentin in SW13c1.1Vim+ cells (D). |
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Gefiltin mRNA is expressed during zebrafish development. At 18 hours post fertilization (hpf), gefiltin expression is found in motor neurons (MN) and the nuclei of the medial longitudinal fasciculus (nMLF) of the midbrain and the nuclei of the tract of the post-optic commissure (nTPOC) of the forebrain (A,B). By 20 hpf embryo, expression is induced in the reticulospinal neurons (RS) of the hindbrain (C,D) and increases by 24 hpf (E,F). New gefiltin expression is found in the retinal ganglion cells (RGC) and the olfactory lobes (OL) at 48 hpf (G). Embryo head 72 hpf (H). Scale bars = 50 mm. |